毛细管区带电泳监测牛胰岛素的去折叠过程  

Monitoring the Process of Bovine Insulin Unfolding by Capillary Zone Electrophoresis

作　　者：董标[1] 董方霆[1] 王京兰[1] 钱小红[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《分析化学》2001年第5期538-541,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 970 5 0 0 3 )

摘　　要：用毛细管区带电泳监测了二硫苏糖醇作用下二硫键还原引起的牛胰岛素去折叠全过程 ,同时用离线的基质辅助激光解吸 /电离飞行时间质谱配合确证。从毛细管电泳谱图能直接观察胰岛素去折叠过程中发生的变化 ,获得蛋白质去折叠信息。结果表明 ,毛细管区带电泳作为监测蛋白质构象变化的一种有效手段 ,方法简便、快速、灵敏度高。Capillary zone electrophoresis (WE) was used to monitor the whole unfolding process of bovine insulin resulted from the dithiothreitol (DTT) reduction. An off-line matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was employed to determine the molecular weight of the reaction products, at the same time to confirm the observation results obtained by CZE. The structural change during the process of bovine insulin unfolding could be observed directly from the electropherogram and information of protein unfolding could be obtained simultaneously. The results indicated that as an effective tool of monitoring the conformational change of protein, the method of CZE was simple, quick, sensitive and lower sample consumption.

关 键 词：毛细管区带电泳 牛胰岛素 基质辅助激光解吸/电离飞行时间质谱 蛋白质 去折叠 

分 类 号：O657.8[理学—分析化学]