红霉素类抗生素电喷雾/飞行时间质谱源内CID研究  

The In-Source CID Study of Erythromycin Analogue Antibiotic in Electrospray Ionization/ Time-of-flight Mass Spectrometry

作　　者：胡守刚[1] 郭寅龙[1] 吕龙[1] 

机构地区：[1]中国科学院上海有机化学研究所,上海200032

出　　处：《质谱学报》2001年第2期10-16,共7页Journal of Chinese Mass Spectrometry Society

基　　金：20 0 0年度上海市自然科学基金资助课题

摘　　要：本文采用电喷雾 /飞行时间质谱法 ,对 5个红霉素类抗生素进行了源内CID研究 ,并以PEG40 0及各样品质子化分子离子 (MH+ )做为内标物 ,对碎片离子进行了准确质量测定 ,确认了这些碎片离子 ,并总结了此类化合物的裂解规律。样品分子含有去氧氨基糖 (desosamine)或红霉糖 (cladinose)两个糖基片段 ,其中去氧氨基糖易于形成带电离子。另外考察了喷管电压 (NozzlePotential)、溶剂条件等对红霉素类抗生素电喷雾源内CID质谱的影响。In source CID of electrospray ionization(ESI)/time of flight mass spectrometry(TOF MS) was applied to five erythromycin analogue antibiotics.Using polyethylene glycol(PEG) and the protonated molecular ion(MH +) as internal reference compounds,the accurate mass of the fragment ions were determined,The fragment ions were elucidated,and their fragmentation patterns were discussed. Erythromycin analogue antibiotics containing structure segments,Desosamine or Cladinose,have more powerful ability to form adduct ions with H + in acid condition.Desosamine can easily form positive ions.The effects of Nozzle Potential and solvents on erythromycin analogue antibiotics of in source CID of electrospray ionization(ESI) were studied.In company with Nozzle Potential rising,the relative abundance of the fragment ions to the protonated molecular ion (MH +) first increases,but after Nozzle Potential reaches to a certain value,it begins to decrease.The higher nozzle potential becomes,the more easily the protonated molecular ion (MH +) methylates with CH 3OH in solvents to form(MH ++CH 3OH-H 2O).And increasing nozzle potential can make the relative abundance of MNa +、MK + rise.Suitable nozzle potential and acid condition were recommended.

关 键 词：电喷雾/飞行时间质谱 源内CID 红霉素类抗生素 PEG内标法 质量测定 

分 类 号：R978.15[医药卫生—药品] O657.63[医药卫生—药学]