荧光素与牛血清蛋白作用的光谱研究与分析应用  

Spectrophotometric Study on the Interaction of Fluorescein and Bovine Serum Albumin

作　　者：王海人[1] 肖忠柏[1] 宋功武[1] 詹红菊[1] 

机构地区：[1]湖北大学分析测试中心,湖北武汉430062

出　　处：《分析测试学报》2001年第4期45-47,共3页Journal of Instrumental Analysis

基　　金：中国分析测试基金资助项目(99 -11)

摘　　要：研究了在生理条件下 ,荧光素与牛血清白蛋白相互作用形成复合物 ,最大吸收峰的波长480nm ,比荧光素红移9nm。该法简便 ,快速,线性范围宽 ,干扰少 ,灵敏度较高 。A spectrophotometric method is presented for the determination of bovine serum albumin(BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 7.4. The complex has a maxmium absorption at 480 nm with 9 nm of red shift compared to fluorescein itself. The linear determination range is between 0 and 100 mg/L for BSA. The method proved to be simple, selective and sensitive for determination of BSA.

关 键 词：荧光素 牛血清蛋白 分光光度法 相互作用 分析方法 

分 类 号：Q51[生物学—生物化学] Q503