高效液相色谱法测定贝类中的软骨藻酸  

High Performance Liquid Chromatographic Determination of Domoic Acid in Shellfish Samples

作　　者：卫锋[1] 程日方[2] 宫静宏[1] 唐守亭[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]大连理工大学,辽宁大连116012

出　　处：《色谱》2001年第3期248-250,共3页Chinese Journal of Chromatography

摘　　要：介绍了用反相高效液相色谱 (RP HPLC)测定贝类中软骨藻酸的方法。样品以V(甲醇 )∶V(水 ) =1∶1的溶液提取 ,经LC SAX强阴离子柱固相萃取净化 ,用RP HPLC定量分析。方法的最小检出限为 0 2 μg/ g ,在 1 0mg/L～ 2 5 0mg/L范围内有良好的线性关系 ,测定结果准确 ,重现性好 ,回收率大于 96 %。Domoic acid in shellfish samples was determined by HPLC with DAD/UV detector at 242 nm Samples were extracted with methanol water followed by clean up of the extracts with strong anion exchange solid phase extraction cartridge(3 mL LC SAX) Zorbax S B C 18 column, 150 mm×4 6 mm i d , and mobile phase of acetonitrile 0.1% aqueous trifluoroacetic acid(13∶87, V/V )were used for the assay The quantitative analysis was performed with external standard The calibration curve of domoic acid was linear in the range of 1 0 m/L 25 0 mg/L and the detection limit was ca 0 2 μg/g

关 键 词：高效液相色谱法 固相萃取 软骨藻酸 贝类 测定 记忆失性欠毒素 

分 类 号：R991[医药卫生—毒理学] R996.3[医药卫生—药学]