高效液相色谱法测定乳膏剂中的联苯苄唑  

Determination of Bifonazole in Cream by High Performance Liquid Chromatography

作　　者：郭兴杰[1] 魏宗有[1] 李发美[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110015

出　　处：《色谱》2001年第3期279-280,共2页Chinese Journal of Chromatography

摘　　要：用高效液相色谱法测定了乳膏剂中联苯苄唑的含量。色谱柱为HypersilODS(2 0 0mm× 4.6mmi.d .,5 μm) ,以 0 .0 8mol/L三乙胺磷酸盐缓冲液 (pH 7.0 ) 乙腈 甲醇 (体积比为 2 0∶10∶70 )为流动相 ,在 2 5 4nm波长处检测。联苯苄唑质量浓度在 8 99mg/L～ 44 96mg/L范围内 ,其浓度与其峰面积的线性关系良好 (r =0 .9998)。该法灵敏度较高 。An accurate method for the determination of bifonazole in cream is described. The analytical column was packed with Hypersil ODS. The mobile phase was a mixture of 0.08 mol/L aqueous triethylamine (TEA) phosphate solution(pH 7.0) acetonitrile methanol (20∶10∶70, V/V ) and the detection wavelength was 254 nm. This method is simple, accurate and can be used for the quality control of bifonazole in cream.

关 键 词：高效液相色谱法 联苯苄 乳膏剂 测定 抗真菌药物 药物分析 

分 类 号：R978.5[医药卫生—药品] R917[医药卫生—药学]