博莱霉素与DNA在镍离子注入修饰电极上的相互作用及其应用  

Study on the Interaction between Bleomycin and DNA at Ni/GCE Ion Implantation Modified Electrode and Its Application

作　　者：胡劲波[1] 黄清泉[2] 李启隆[1] 

机构地区：[1]北京师范大学化学系, [2]中国药品生物制品检定所,

出　　处：《化学学报》2001年第6期836-841,共6页Acta Chimica Sinica

基　　金：国家自然科学基金，高等学校博士学科点专项科研项目

摘　　要：博莱霉素(BLM)在0.1 mol/L HOAc-NaOAc缓冲溶液(pH4.62)中,在Ni/GCE离子注入修饰电极上有一灵敏的还原峰,峰电位为-1.16 V(vs.SCE),峰电流与BLM浓度有关.用线性扫描和循环伏安法研究体系的行为表明,体系为具有加速作用的不可逆过程.是注入的Ni加速BLM的还原.引入DNA后,BLM的峰电位为-1.15 V(vs.SCE),与未加入DNA前几乎完全一致;只使峰电流降低,形成一种非电活性的结合物.求得该结合物的结合比为BLM:DNA=3:1,结合常数为β=3.16×1016.用线性扫描和循环伏安法,并辅以紫外可见光谱法等手段研究表明,电极过程仍为不可逆过程,与未加入DNA时一样.加入DNA后,BLM的峰电流降低,据此,可用于DNA的测定,回收率在96.8～103.9%之间.With a Ni/GCE ion implantation modified electrode in 0.1 mol/L HOAc - NaOAc solution ( pH 4.62), a sensitive reduction peak of bleomycin( BLM) was obtained by linear - sweep voltammetry. The peak potential is -1.16 V(vs. SCE). The peak current is proportional to the concentration of BLM. The electrochemical behavior of the system was also studied by cyclic voltammetry. The experimental results depict that the electrode process at Ni/GCE is irreversible and the reduction of BLM is accelerated obviously. Measurements of AES show that Ni is surely implanted into the surface of GCE. After adding DKA to the solution containing BLM, the peak potential is almost no change, but the peak current of BLM is decreased. The interaction between BLM and DNA at a Ni/GCE modified electrode was studied by linear sweep and cyclic voltammetry. An inactive complex of BLM with DNA was formed and resulted in a decrease of BLM concentration as well as the reduction current. The composition of the complex is estimated as BLM:DNA = 3: 1 and the combination constant is calculated as 3.16 x 10(16). Its electrochemical behavior is the same as that in the absence of DNA. The decrease in the peak current is proportional to DNA concentration and can be used to the determination of the concentration of DNA.

关 键 词：镍离子 化学修饰电极 博莱霉素 离子注入 伏安行为 表面分析 抗癌药物 相互作用 DNA 

分 类 号：R979.1[医药卫生—药品] O646.5[医药卫生—药学]