肥皂草素Saporin-S6二级结构的圆二色光谱及激光喇曼光谱法表征  

A CD and FT-Raman Study on the Secondary Structure of Saporin-S6

作　　者：王永亭[1] 黄品伟[2] 李正强[2] 徐菲 孟庆繁[1] 杨谊[3] 史学惠[3] 李青山[1] 

机构地区：[1]吉林大学生命科学学院,长春130023 [2]吉林大学理论化学研究所超分子结构及谱学教育部开放实验室,长春130023 [3]吉林省药品检验所

出　　处：《高等学校化学学报》2001年第7期1117-1119,共3页Chemical Journal of Chinese Universities

基　　金：清华大学生物膜与膜生物工程国家重点实验室资助

摘　　要：Saporin S6 is purified from the seeds of Saponaria officinalis and its CD and FT Raman spectra are determined. From the CD spectra of fresh protein aqueous and the same aqueous that was laid aside for 90 days, it is proved that saporin S6 has unusual stability. Throughout the FT Raman spectra of saporin S6, we work out that the α helix of saporin S6 is 26%, while β fold is 37%, and random coil is 37%.Saporin S6 is purified from the seeds of Saponaria officinalis and its CD and FT Raman spectra are determined. From the CD spectra of fresh protein aqueous and the same aqueous that was laid aside for 90 days, it is proved that saporin S6 has unusual stability. Throughout the FT Raman spectra of saporin S6, we work out that the α helix of saporin S6 is 26%, while β fold is 37%, and random coil is 37%.

关 键 词：肥皂草素 二级结构 喇曼光谱 圆二色谱法 蛋白质 表征 植物药 Saporin- S6 

分 类 号：R284.1[医药卫生—中药学] Q518.1[医药卫生—中医学]