四氨基铝酞菁作为过氧化物酶模拟酶新型红区荧光底物的研究  

Studies on Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Substrate for Mimetic Peroxidase

作　　者：陈小兰[1] 李东辉[2] 杨黄浩[1] 朱庆枝[1] 郑洪[1] 许金钩[1] 

机构地区：[1]厦门大学化学系现代分析科学教育部重点实验室 [2]厦门大学抗癌研究中心,厦门361005

出　　处：《高等学校化学学报》2001年第7期1120-1121,共2页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 9775 0 2 1)

摘　　要：Tetra substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red region fluorescent substrate for the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear range of 0.0-3.0×10 -7 mol/L H 2O 2 with a detection limit of 3.7×10 -9 mol/L. The feasibility of TAAlPc as a new promising red region substrate in practical application has been proven in the determination of H 2O 2 in rainwater.The proposed method can largely minimize the interference that results from background fluorescence or scattering light and has a high analytical sensitivity.Tetra substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red region fluorescent substrate for the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear range of 0.0-3.0×10 -7 mol/L H 2O 2 with a detection limit of 3.7×10 -9 mol/L. The feasibility of TAAlPc as a new promising red region substrate in practical application has been proven in the determination of H 2O 2 in rainwater.The proposed method can largely minimize the interference that results from background fluorescence or scattering light and has a high analytical sensitivity.

关 键 词：氯化血红素 四氨基铝酞菁 过氧化氢 过氧化物酶 模拟酶 痕量分析 红区荧光底物 荧光分析 

分 类 号：Q554.6[生物学—生物化学] O657.3[理学—分析化学]