尿毒症中分子毒物的分离及飞行时间质谱分析  

Separation and Analyses of Uremic Middle Molecules by Chromatography and Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

作　　者：储结根[1] 袁直[1] 吴强[1] 何炳林[1] 

机构地区：[1]吸附分离功能高分子材料国家重点实验室南开大学高分子化学研究所,天津300071

出　　处：《高等学校化学学报》2001年第7期1166-1168,共3页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展规划项目基金 (批准号 :G19990 6 470 7);国家自然科学基金 (批准号 :5 98730 11);高等学校博士学科点专项基金 (批准号 :980 0 5 5 0 3);天津市自然科学基金 (批准号 :9836 0 9711

摘　　要：Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic serum and urine were less than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 8 to 9 subpeaks at 230 nm by anion exchange chromatography. One of these subpeaks, A 3, was detected in uremic serum and normal urine but undetectable in uremic urine. After a gel permeation chromatography with bidistilled water as eluent for desalting, subfraction A 3 was seperated into two parts designated A 3 Ⅰ and A 3 Ⅱ in order. The results of MALDI TOF MS revealed that the two peaks from both samples were identical respectively, fraction A 3 Ⅰ contained three kinds of components with molecular weight 839.69, 1 007.94 and 2 015.16 and fraction A 3 Ⅱ consisted of other three kinds of components with molecular weight 873.69, 1 106.67 and 1 680.28.Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic serum and urine were less than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 8 to 9 subpeaks at 230 nm by anion exchange chromatography. One of these subpeaks, A 3, was detected in uremic serum and normal urine but undetectable in uremic urine. After a gel permeation chromatography with bidistilled water as eluent for desalting, subfraction A 3 was seperated into two parts designated A 3 Ⅰ and A 3 Ⅱ in order. The results of MALDI TOF MS revealed that the two peaks from both samples were identical respectively, fraction A 3 Ⅰ contained three kinds of components with molecular weight 839.69, 1 007.94 and 2 015.16 and fraction A 3 Ⅱ consisted of other three kinds of components with molecular weight 873.69, 1 106.67 and 1 680.28.

关 键 词：血清 尿液 分子量 分析 分离 尿毒症 中分子物质 凝胶色谱 离子交换色谱 飞行时间质谱 

分 类 号：R695[医药卫生—泌尿科学] R446.1[医药卫生—外科学]