气相色谱-电子捕获检测法测定人尿中α-羟基三唑仑及α-羟基阿普唑仑  

Determination of α-Hydroxytriazolam and α-Hydroxyalprazolam in Human Urine by Gas Chromatography/Electron-Capture Detector

作　　者：姜兆林[1] 谭家镒[1] 姚丽娟[1] 邢丽梅[1] 蒋泽良[1] 何国标[1] 

机构地区：[1]中国刑警学院法医系,沈阳110035

出　　处：《分析化学》2001年第6期642-645,共4页Chinese Journal of Analytical Chemistry

摘　　要：建立了尿中三唑仑的代谢物α－羟基三唑仑和阿普唑仑的代谢物α－羟基阿普唑仑的气相色谱－电子捕获检测法。代谢物用Ｎ，Ｏ－双三甲基硅烷基－三氟乙酰胺（ＢＳＴＦＡ）进行三甲基硅烷衍生化后进行检测。尿中代谢物的检出限约为１μｇ／Ｌ（Ｓ／Ｎ＝３），至少可检出口服治疗量药物人体２４ｈ之内排泄尿中的代谢物，满足麻醉后犯罪案件中药物检验的要求。A method was developed to assay α-Hydroxyltriazolam and α-Hydroxyalprazolam, which are the major metabolites of triazolam and alprazolam respectively, in human urine. After addition of 2-hydroxyflurazepam (interal standard) and hydrolysis with β-glucuronidase, the hydroxy-metabolites were extracted with hexane-dichloromethane (1: 1) at pH 10.8, then were derivated with (BSTFA). The analysis was performed on a HP-5 capillary column with electron-capture detector. The detection limits of analysis in urine were about 1μg/L. The method was successfully applied to urine specimens collected from healthy human volunteers who ingested 0. 5 mg of triazolam or 0.8 mp alprazolam. The method was enough sensitive to assay urine specimen excreted at 24 h by volunteers after taking the medicine.

关 键 词：三唑仑 阿普唑仑 Α-羟基三唑仑 α-羟基阿普唑仑 气相色谱法 尿 药物检测 麻醉抢劫案 

分 类 号：O657.7[理学—分析化学]