灿烂甲酚蓝共振光谱散射法测定脱氧核糖核酸  

Resonance Light Scattering Method for the Determination of Deoxyribonucleic Acid with Brilliant Crystal Blue

作　　者：刘晨[1] 陈小明[1] 

机构地区：[1]湘潭大学化学化工学院,湘潭411105

出　　处：《分析化学》2001年第6期685-688,共4页Chinese Journal of Analytical Chemistry

基　　金：湖南省自然科学基金资助项目(No.00JJY2084)

摘　　要：研究了灿烂甲酚蓝与脱氧核糖核酸（ＤＮＡ）作用的共振光散射光谱，在ｐＨ＝１０．８～１１．５的范围内，ＤＮＡ的加入导致灿烂甲酚蓝共振光散射的增强，在３４７ｎｍ处，存在一共振光散射增强峰，其强度与ＤＮＡ的浓度呈线性关系，据此建立了一种测定ＤＮＡ的共振光谱散射法。该方法的线性范围为８０～１０００μｇ／Ｌ，检出限为２３．３μｇ／Ｌ。The resonance light scattering (are) spectra of brilliant crystal blue with deoxyribonucleic acid have been studied. The RLS of brilliant crystal blue is greatly enhanced by deoxyribonucleic acid in pH range of 11. 0 - 11. 5. There is a resonance light scattering peak at 347 nm, and the enhanced intensity of RLS at this wavelength is proportional to the concentration of deoxyribonucleic acid. So a method of RLS for the determination of deoxyribonucleic acid is established. The linear range of the calibration graph is 80 - 1000μg/L. The detection limit is 23. 3 μg/L. This method is simple, rapid and has been applied to the determination of deoxyribonucleic acid in mixed samples with satisfactory results.

关 键 词：灿烂甲酚蓝 共振光散射法 脱氧核糖核酸 DNA 测定 光谱法 

分 类 号：O657.3[理学—分析化学]