性病艾滋病感染者HIV-1相关基因多态性分析及其意义  

Polymorphisms and Implications of HIV-1 Relevant Alleles in Patients with Sexually-Transmitted Diseases and HIV-1 Carriers

作　　者：洪卫国[1] 王哲[2] 王福生[1] 崔为国[2] 金磊[1] 韩卫国[2] 侯静[1] 雷周云[1] 刘明旭[1] 

机构地区：[1]解放军第302医院生物工程研究室,北京100039 [2]河南省卫生防疫站

出　　处：《中国皮肤性病学杂志》2001年第4期259-261,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助(397706830)

摘　　要：目的 研究ＨＩＶ－１相关等位基因（CCR5、CCR2b和SDF1在性病、艾滋病感染人群中的分布，为分析这些宿主遗传因素在ＨＩＶ－1感染、艾滋病发病进程中的作用提供理论依据。方法 收集性病、艾滋病感染者的血液标本，提取基因组ＤＮＡ，经PCＲ或ＰＣＲ-ＲＦＬＰ分析，计算突变基因频率。结果ＣＣR2ｂ-６４Ｉ和ＳＤＦＩ－３'Ａ的突变频率较高。２１５例性病患者和７５例艾滋病感染者ＣＣＲ2ｂ-６４Ｉ的突变频率分别为１８．６０％和１５．３３％，ＳＤＦＩ－３'Ａ的突变频率分别为２４.４２％和２３．３３％。但ＣＣＲ５△３２的突变频率较低（０．２３％），而 ＣCR5m303等位基因突变率为0,这与中国普通汉族人和该地区普通汉族人的结果一致。结论 提示在遗传机制上艾滋病对中国人构成的威胁比西方人更大。Objective To study the distribution of CCR△532, CCR5m3O3, CCR2b-64 I and SDF1-3 'A alleles in the patients with STD and HIV- 1 carriers, and to evaluated the role of these alleles in the development of the diseases. Methods The genomic DNA from 290 patients with STD(215) and HIV-1 carriers (75) in Henan province of China were purified from whole blood samples by QIAmp DNA Blood Mini Kit and identified by means of PCR or PCR-RFLP analyses. Results The study showed that the mutation frequency of CCR2b-64I alleles was 18.60% and 15.33% respectively in patients with STD and HIV-1carriers, SDF1-3 'A was 24.42% and 23. 33% . The results are similar to that in Chinese normal population,which possess a characteraztion of very lower frequency of CCR5△32and CCR5m3O3,and higher frequency of CCR2b-64I and SDF1-3' A alleles.Conclusion The results suggested Chinese patients with STD may be highly susceptive to HIV-l infection, and the significances of above mutant HIV-1-related alleles need to be evaluated in HIV-1 carriers.

关 键 词：HIV-1 遗传易感性 基因多态性 协同受体 性传播疾病 

分 类 号：R512.91[医药卫生—内科学] R759[医药卫生—临床医学]