男性泌尿生殖道沙眼衣原体感染的两种免疫检测方法的比较  

Comparison of Two Immunoassays for the Detection of Chlamydia Trachomatis in Male Urethral and Urine Specimens

作　　者：黄东辉 黄树杰 程文海 卢和琨 谢礼豪 谭仲楷 

机构地区：[1]江门市皮肤病防治所,广东江门529000

出　　处：《中国皮肤性病学杂志》2001年第4期262-263,282,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的 比较 ＶＩＤＡＳ衣原体试验（ＶＩＤＡＳ ＣＨＬ）和“立明”衣原体快速法(C-C法）的敏感性和特异性。方法 同时用细胞培养（Ｔc）、聚合酶链反应技术（PCR）、ＶＩＤＡＳ ＣＨＬ、Ｃ-Ｃ法对 ２３０例男性患者进行了衣原体检测。结果以 Ｔc为金标准，ＶＩＤＡＳ ＣＨＬ、C-Ｃ法的敏感性和特异性分别是 ９７. ９％、９５. ６％，８５．４％、９９．５％，两者间差异无显著性；而以扩大金标准来比较，它们的敏感性和特异性分别是９６．２％、９８．３％，７９．２％、１００％，在敏感性方面两者之间差异有显著性。结论 ＶＩＤＡＳ ＣＨＬ是一种检测沙眼衣原体的自动酶联免疫荧光方法，由于其阳性和可疑结果可以经阻抑试验证实，故保证了结果的准确性，而且其敏感性更高，显示了比Ｃ-Ｃ法更优的应用前景。Objective To compare the sensitivity and specificity of VIDAS CHL with that of clearview chlamydia MF(C-C MF) . Methods Tissue culture (Tc) , PCR, VIDAS CHL and C-C MF were used to detect chlamydia antigens in 230 male patients. Results When compared to Tc, the sensitivity, specificity were 97.9% ,95.6% for VIDAS CHL, and 85.4% ,99. 5% for C-C MF, respectively, there was no statistical difference between VIDAS CHL and C-C MF, but in comparison with expanded gold standerd,the sensitivty,specificity were 96.2% ,98.3% for VIDAS CHL,and 79.2% ,100% for C-C MF,there was obvious difference in sensitivity between them. Conclusion Because the VIDAS CHL is an automated enzyme-linked immunofluorescence assay for the the direct detection of chlamydia antigens, positive and equivocal results are confirmed with a blocking assay,and it was more sensitive than C-C MF,so the VIDAS CHL is an attractive alternative.

关 键 词：沙眼衣原体 免疫检测方法 男性 泌尿生殖道感染 

分 类 号：R691.3[医药卫生—泌尿科学]