反复自然流产病人细胞因子水平测定  

Detection of Cytokines in Women with Recurrent Spontaneous Abortion

作　　者：王艾丽[1] 党洁民[2] 江舟[1] 王娟[1] 武建国[1] 

机构地区：[1]南京军区南京总医院全军医学检验中心,江苏南京210002 [2]南京军区南京总医院妇产科,江苏南京210002

出　　处：《中华男科学杂志》2001年第3期164-166,共3页National Journal of Andrology

摘　　要：目的 :探讨不明原因反复自然流产 (RSA)病人PBMC产生IFN γ、TNF α、IL 4和血清sIL 2R水平的变化及意义。　方法 :应用酶联免疫吸附法 (ELISA)测定了 36例RSA病人、15例正常妊娠、13例经产妇以及 8例配偶白细胞免疫治疗者外周血中单个核细胞经PHA刺激后培养上清中 ,IFN γ、TNF α和IL 4以及血清中sIL 2R水平。　结果 :RSA病人PBMC产生的IFN γ为 (0 .5 9± 0 .2 6 )ng/ml,TNF α为 (1.11± 0 .5 6 )ng/ml,较对照者显著增高 (P <0 .0 5 )。经配偶白细胞免疫治疗者 (成功妊娠 )的IFN γ、TNF α水平明显低于治疗前。RSA病人妊娠后再次流产血清sIL 2R显著增高。　结论 :RSA病人某些细胞因子产生异常是妊娠的不利因素 ,sIL 2R增高与先兆流产有关。Objectives: To investigate the changes and significance of interferon γ (IFN γ), tumor necrosis factor α (TNF α), interleukin 4 (IL 4) and soluble interleukin 2 receptor (sIL 2R) levels in women with undefined recurrent spontaneous abortion (RSA). Methods: IFN γ、TNF α and IL 4 produced by PHA induced PBMC in the supernatant and sIL 2R in the serum were measured by ELISA in women with undefined RSA ( n =36), undergoing normal pregnancy ( n =15), having history of a normal pregnancy ( n =13) and leukocyte immunization (n =8). Results: Both the levels of IFN γ [(0.59±0.26)ng/ml] and TNF α [(1.11±0.56)ng/ml] in women with RSA were significantly higher than those in other two control groups. After the immunological treatment with leukocytes, the levels of IFN γ and TNF α in the aborters reduced significantly. The levels of sIL 2R in sera from women with RSA were significantly increased. Conclusions: The abnormal elevation of IFN γ and TNF α in women with RSA is unfavourable for normal pregnancy while the increase of sIL 2R may be associated with threatened abortion.

关 键 词：反复流产 免疫疗法 INF-Γ TNF-α IL-4 SIL-2R 

分 类 号：R714.21[医药卫生—妇产科学]