烷化溶血磷脂作为骨髓净化药物的实验研究  

STUDY OF ALP AS BONE MARROW PURGING AGENT

作　　者：黄慧芳[1] 陈志哲[1] 卓光生[1] 王少元[1] 

机构地区：[1]福建医科大学附属协和医院福建省血液病研究所,福州350001

出　　处：《中国现代医学杂志》2001年第6期26-27,29,共3页China Journal of Modern Medicine

摘　　要：目的 :探讨烷化溶血磷脂 (ALP)作为骨髓净化药物的潜在应用价值。方法 :克隆形成实验测定HL6 0细胞生长 ;正常粒 -单核系造血祖细胞 (CFU -GM )集落产率的变化检测ALP对正常造血祖细胞的影响 ;RT -PCR检测bcl- 2mRNA表达 ;流式细胞术检测Bcl- 2蛋白表达。结果 :ALP作用后 ,HL6 0细胞的克隆形成率明显下降 (P <0 .0 1) ;ALP对CFU -GM的影响不显著 ,2 0 μg/ml预处理 2 4h ,CFU -GM集落产率才被抑制 (P <0 .0 1) ;ALP不影响bcl- 2基因在mRNA及蛋白水平的表达。结论 :ALP具有抑制白血病细胞增殖的作用 ,但对正常造血细胞无显著毒性 ,在白血病病人自体骨髓体外净化中有良好的应用前景。Objective:To explore the potency of ALP as a purging agent in autologous bone marrow transplantation (ABMT).Methods:Proliferative potential of HL60 cells was measured by colony formation assays.ALP'S antiproliferative effects on normal hematopoietic progenitors were evaluated by assays of CFU-GM formation.The mRNA level of bcl-2 was determined by reverse transcription-polymerase chain reaction (RT-PCR).The expression of bcl-2 oncoprotein was analyzed by flow cytometry.Results:ALP significantly inhibited HL60 cells proliferation.15μg/ml ALP treatment of HL60 cells for 24h could eliminate 100% of conogenic HL60 cells,whereas,the CFU-GM were relatively spared.Bcl-2 expression in HL60 cells treated by ALP did not decrease as measured by RT-PCR or by flow cytometry.Conclusions:ALP could exert selective antineoplastic effects.ALP may be a promising purging agent in ABMT.

关 键 词：抗肿瘤药物 烷化熔血磷脂 HL60细胞 药理 

分 类 号：R979.11[医药卫生—药品]