放射复合伤口中Bax和Bcl-2蛋白表达与细胞凋亡的关系研究  

Relationship between the expressions of Bax and Bcl2 proteins and apoptosis in radiation combined wound of rats

作　　者：崔玉芳[1] 夏国伟[1] 付小兵[2] 杨红[1] 彭瑞云[1] 高亚兵[1] 王德文[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]解放军第三○四医院创伤研究中心

出　　处：《中国危重病急救医学》2001年第7期404-406,共3页Chinese Critical Care Medicine

基　　金：全军"九.五"科研基金资助项目 (No.98Q0 86);国家"973"科研基金资助项目 (No.G19990 5 42 0 4)

摘　　要：目的 :研究 Bax和 Bcl 2蛋白在放射复合伤口愈合中的表达及其与细胞凋亡的关系。方法 :用原位末端标记 (TU NEL)法检测细胞凋亡 ,用免疫组织化学方法检测 Bax、Bcl 2蛋白表达。结果 :(1)与单伤组比较 ,照射后细胞凋亡的变化具有出现较早、频度较高、消失推迟 3个显著特点 ,可能是导致辐射延迟伤口愈合的重要原因。 (2 )观察伤口愈合过程中 Bax、Bcl 2蛋白表达及其与细胞凋亡的关系 ,发现随着细胞凋亡的增加 ,Bax蛋白的表达明显增强 ,并且与细胞凋亡发生频度具有良好的相应关系。而 Bcl 2蛋白水平在细胞凋亡达高峰时呈明显下降趋势 ;当凋亡明显下降时 ,Bcl 2蛋白表达增加 ,并逐渐恢复其正常水平。结论 :Bax和 BclObjective:To study the relationship between the expressions of Bax and Bcl2 proteins and apoptosis in radiation combined wound of rats.Methods:Apoptosis and Bax and Bcl2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical methods.Results:(1)It was shown that apoptosis manifested three typical characteristics,namely early occurrence,high frequency and delayed disappearance after radiation,when compared with those of single wound group.This characteristics might explain the delayed wound healing caused by radiation.(2) The expression of Bax protein increased evidently with the increment of apoptosis,and it showed a good correlation with apoptosis in the process of wound healing.The expression of Bcl2 protein decreased obviously as the apoptosis reached the beak,and it showed an ascending tendency up to normal level when the apoptosis decreased distinctively.Conclusions:Bax and Bcl2 proteins played an important role in the regulation of apoptosis in the process of healing of combined wound in rats.

关 键 词：放射复合伤口 BAX蛋白 BCL-2蛋白 细胞凋亡 

分 类 号：R818.74[医药卫生—放射医学]