神经生长因子对损伤脊髓组织一氧化氮生成的影响  

Effects of nerve growth factor on nitric oxide production after spinal cord injury in rats

作　　者：王道新[1] 曹晓建[1] 汤长华[1] 

机构地区：[1]南京医科大学第一附属医院骨科,江苏南京210029

出　　处：《中国危重病急救医学》2001年第7期417-419,共3页Chinese Critical Care Medicine

基　　金：国家自然科学基金资助项目 (No.3 980 0 176)

摘　　要：目的 :探讨神经生长因子 (NGF)对脊髓损伤保护作用的机制。方法 :采用 Allen's方法以 0 .1N×2 .5 cm致伤大鼠 T8脊髓 ,插入蛛网膜下腔导管 ,于术后即刻及 2、4、8、12和 2 4小时各注入 NGF溶液 ,并与生理盐水组和正常对照组作比较。采用免疫组织化学法检测神经元固有型一氧化氮合酶 (nc NOS)在脊髓中的表达。结果 :与正常对照组比较 ,大鼠伤后脊髓前角运动神经元出现 nc NOS蛋白异常表达 ,NGF组 nc NOS蛋白异常表达较生理盐水组明显减少。结论 :NGF能通过抑制脊髓损伤后 nc NOS的异常表达来抑制一氧化氮(NO)过多释放所致的神经毒性作用 。Objective:To explore the protective mechanisms of nerve growth factor (NGF) in spinal cord injury(SCI).Methods:SCI in Wistar rats was created with Allen′s method by a 10 N×2 5 cm impact on the posterior T8 spinal cord.NGF was introduced into the subarachnoid space of the rats in treatment group immediately and 2,4,8,12 and 24 hours after the injury.The neuronal constitutive nitric oxide synthase (ncNOS) protein levels in rat spinal cord were assayed with immunohistochemical methods.Results:Abnormal expression of ncNOS was induced in spinal ventral horn motorneuron in injured rats,as compared with that in control group.The levels of ncNOS protein in NGF group were significantly lower as compared with that in normal saline group.Conclusions:NGF can protect spinal cord from injury in vivo.One of mechanisms is that NGF inhibits abnormal expression of nitric oxide synthase (NOS),thus the neurotoxicity of excessive nitric oxide(NO) is abated .

关 键 词：脊髓损伤 神经生长因子 一氧化氮 免疫组织化学 

分 类 号：R651.2[医药卫生—外科学]