正常皮肤和瘢痕组织表达β1整合素与系列角蛋白的比较性研究  

Different expression of stem cell markers in scars from children and adults

作　　者：孙晓庆[1] 付小兵[1] 孙同柱[1] 李建福[1] 陈伟[1] 盛志勇[1] 

机构地区：[1]解放军第三○四医院,北京100037

出　　处：《中国危重病急救医学》2001年第7期420-422,共3页Chinese Critical Care Medicine

基　　金：国家重点基础研究发展规划项目基金资助(No.G19990 5 42 0 4);国家杰出青年科学基金资助项目 (No.3 95 2 5 0 2 4)

摘　　要：目的 :研究幼儿与成人大面积重度烧伤后瘢痕组织表皮细胞表达 β1整合素及系列角蛋白的特征与规律 ,以确定表皮干细胞及短暂扩充细胞分布、数量的差异及这些改变与瘢痕愈合关系。方法 :分别取 4～ 10岁幼儿及 35～ 5 1岁成人 2组健康皮肤及大面积深度烧伤后瘢痕组织。采用免疫组织化学方法检测表皮干细胞、短暂扩充细胞特异表达的β1整合素和角蛋白 19(K19)及分化表皮细胞表达的角蛋白 14与 10 (K14和 K10 )。结果 :瘢痕组织表皮基底层表达β1整合素与 K19的阳性细胞数较健康皮肤明显减少 ,阳性强度降低 ;瘢痕组织表皮中表达 K14的阳性细胞仅位于表皮底部 2～ 3层 ,明显少于健康皮肤 ;而 K10表达阳性细胞则较健康皮肤分布广泛。结论 :瘢痕组织表皮基底层具有增殖能力的表皮干细胞和短暂扩充细胞明显少于健康皮肤 ,且瘢痕组织表皮干细胞的分化过程与健康皮肤不同 ,处于有丝分裂后细胞阶段的细胞比例降低 ,而终末分化细胞的比例明显增高。瘢痕组织表皮的增殖能力下降、细胞的分化行为紊乱可能是瘢痕组织表皮结构与功能改变。Objective:To study the expression characteristics of epidermal stem cells,transit amplifying cells,postmitotic cells and terminallydifferentiated cells in normal skin and scar epidermis from children and adults,and to explore the mechanisms of the ability of selfrenewal and wound healing in scar.Methods:β1 integrin and keratin 19 (K19) were used as the biochemical markers for stem cell and transit amplifying cell identification,and keratin 14 (K14) and keratin 10 (K10) were used as the markers for postmitotic cells and terminallydifferentiated cells,respectively.Normal skin and scar were taken from children (410 years) and adults (3551 years).Immunohistochemistry was used.Results:As for the immunostainings of tissue sections from children and adults,the positive expression cells of β1 integrin and K19 in scar were less than those in normal skin.Whereas,the proportion of K14 positive cells were decreased and K10 expression cells were observed in all epidermal cells except basal layer in scar sections.Conclusions:These results indicated that stem cells and transit amplifying cells in scar epidermis were less than those in normal skin,and proportions of postmitotic cells and terminallydifferentiated cells were abnormal which maybe a reason of the decreasing ability of selfrenewal and wound healing in scar.

关 键 词：烧伤 瘢痕 表皮干细胞 Β1整合素 角蛋白 

分 类 号：R644[医药卫生—外科学]