狼疮性肾炎患者外周血淋巴细胞亚群及活化状态的研究  

Change of subset and activated state of lymphocytes from patients with lupus nephritis

作　　者：左巍[1] 马东初[2] 刘永海[1] 左伟[3] 陈红[1] 杨志蕃[1] 王丽娜[1] 

机构地区：[1]沈阳军区总医院肾脏科,辽宁沈阳110016 [2]沈阳军区总医院医学实验科,辽宁沈阳110016 [3]沈阳军区总医院检验科,辽宁沈阳110016

出　　处：《细胞与分子免疫学杂志》2001年第4期348-349,共2页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨狼疮性肾炎(LN)患者淋巴细胞亚群的变化及所处的不同状态在LN发病机制中的作用。方法采用双标染色技术结合流式细胞术，对32例LN进行研究。结果①LN患者CD3＋CD4＋细胞的百分率明显下降，CD3＋CD8＋细胞的百分率明显增高，CD3＋CD4＋/CD3＋CD8＋的比例∨1(与对照组相比较,P∨0.05)。②LN患者CD45RA＋细胞、CD4＋CD45RA＋细胞、CD4＋CD45RO＋细胞和CD4＋HLA-DR＋细胞的百分率下降(与对照组相比较,P∨0.05或P∨0.001), HLA-DR＋细胞、CD3＋HLA-DR＋细胞、CD19＋HLA-DR＋细胞和CD19＋CD43＋细胞的百分率明显增高(与对照组相比较，P∨0.05或P∨0.01)。结论LN患者免疫功能的紊乱与淋巴细胞活化状态及其亚群之间的平衡失调密切相关。：Aim To explor the distribution of lymphocytic subset and activated state of lymphocyte in peripheral blood from the patients with lupus nephritis(LN). Methods The immunophenotypes in peripheral blood lymphocytes of 32 LN patients and 40 healthy volunteers were analysed by flowcytometor with double-labeling using anti-CD3，anti-CD4，anti-CD8，anti-CD19，anti-CD43，anti-CD45RA，anti-CD45RO anti-HLA-DR antibodies. Results ①The percentage of CD3＋CD4＋subset decreased significantly, meanwhile the percentage of CD3＋CD8＋subset increased; the ratio of CD3＋CD4＋/CD3＋CD8＋subset was less than 1(P∨0.05 in contrast to control).②The percentages of CD45R＋, CD4＋CD45RA＋, CD4＋CD45RO＋and CD4＋HLA-DR＋subsets in LN patients decreased (P∨0.05 or P∨0.01 respectively, as compared with control group); the percentages of HLA-DR＋, CD3＋HLA-DR＋, CD19＋HLA-DR＋subset and CD19＋CD43＋subset increased(P∨0.05 or P∨0.01 respectively, as compared with control group). Conclusion The dysfunction of immune system in the LN patients may be related to the activation of lymphocytes and imbalance among the lymphocytic subsets.

关 键 词：狼疮性肾炎 淋巴细胞亚群 活化淋巴细胞 

分 类 号：R593.242[医药卫生—内科学]