氧自由基对严重烧伤后红细胞免疫粘附功能的影响  

Influence of oxygen-free radical to erythrocyte immune adherent function after severe burns

作　　者：陈晓东[1] 吴伯瑜[1] 黄逢元[1] 江琼[1] 

机构地区：[1]福建医科大学附属协和医院省烧伤研究所,福建福州350001

出　　处：《细胞与分子免疫学杂志》2001年第4期389-391,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨氧自由基对烧伤后红细胞免疫粘附功能的影响。方法于伤后1 d、3 d、5 d、7 d、14 d、21 d、28 d和35d，观察RBC-C3b受体花环形成率(RC3b RR)和RBC-IC花环形成率(RICR)，以及SOD、Lpo-MDA、CAT、GSH-px和GSH水平的变化，并进行相关性分析。结果①伤后RC3bRR显著下降，RICR显著升高(P∨0.05～0.001)，但4wk后逐渐恢复至正常。②伤后SOD、CAT、GSH-px和GSH的水平显著下降，Lpo-MDA的水平则显著上升(P∨0.05～0.001)，至少伤后3wk才恢复到正常水平。③RC3bRR与SOD、CAT、GSH-px和GSH的水平呈显著的正相关(r值依次分别为:0.601，0.799，0.715和0.597)，与Lpo-MDA的水平呈显著的负相关(r=-0.564)；RICR与SOD、CAT、GSH-px和GSH的水平也呈显著的负相关(r值依次分别为:-0.359、-0.577、-0.420和-0.427)，与Lpo-MDA的水平则呈显著的正相关(r=0.433)。结论红细胞膜极易产生脂质过氧化作用，烧伤后自由基损伤的程度，可直接影响红细胞膜的功能，特别是红细胞免疫粘附功能。在烧伤治疗过程中，提示减少机体自由基的产生，提高患者红细胞免疫粘附功能是十分必要的。Aim To investigate the influence of oxygen-free radical to erythrocyte immune adherent function after severe burns. Methods The erythrocyte's immune adherent function (RC3bRR and RICR) and the levels of SOD, Lpo-MDA, CAT, GSH-px and GSH were detected on 1, 3, 5, 7, 14, 21, 28 and 35 days after severe burns, and then their correlations were analyzed. Results The rosette forming rate of erythrocyte C3b receptor (RC3bRR) in post-burn group was obviously lower than that in normal control group (P∨0.05～0.001), and erythrocyte immune complex rosette forming rate (RICR) of the burned patients was obviously higher than that of the healthy group(P∨0.001), but they gradually returned to normal four weeks after burn. Compared with healthy control group, the levels of SOD, CAT, GSH-px and GSH markedly declined(P∨0.05～0.001), while Lpo-MDA level notably increased(P∨0.05～0.001), but their recovery needed at least three weeks. RC3bRR was positively correlated with the levels of SOD, CAT, GSH-px and GSH, and was negatively correlated with Lpo-MDA. Correlation of RICR was contrary to that of RC3bRR. Conclusion The degree of the free radical injury may directly influence the erythrocyte membrane function, especially the erythrocyte immune adherent. It is necessary to reducing the production of free radicals and enhacing patient's erythrocyte immune function during burn treatment.

关 键 词：烧伤 红细胞免疫粘附功能 氧自由基 

分 类 号：R644[医药卫生—外科学]