光培养和暗培养绿草履虫中同工酶的比较研究  

A Study on the Isoenzymes of Paramecium bursaria Cultured in Light and Dark

作　　者：张许枚[1] 田沁[1] 顾福康[1] 

机构地区：[1]华东师范大学生物系,上海200062

出　　处：《华东师范大学学报（自然科学版）》2001年第2期78-83,共6页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金!( 39970 0 88);高等学校博士学科点专项科研基金资助课题

摘　　要：作者应用聚丙烯酰胺凝胶电泳技术分析比较了光培养绿草履虫和暗培养绿草履虫中酯酶、酸性磷酸酶、苹果酸脱氢酶和乳酸脱氢酶四种同工酶的组分及含量的变化。除了对乳酸脱氢酶未检出酶蛋白条带外 ,其它三种酶的电泳图谱都显示暗培养草履虫的同工酶组成条带多、含量高。PAGE was used to analyze and compare the variation of composition and content of four isoenzymes , esterase, acid phosphatase, malate dehydrogenase (MDH) and lactate dehydrogenase(LDH), of Paramecium bursaria which cultured in light and dark . Except LDH was not checked up band, the electrophoresis results of other three isoenzymes indicated that the isoenzymes of dark cultivated P. bursaria had more bands and higher content .From these results it can be concluded that the symbiotic Chlorella probably effect the gene expression of the host cell.

关 键 词：绿草履虫 共生小球藻 同工酶 光培养 暗培养 酯酶 酸性磷酸酶 

分 类 号：Q959.117[生物学—动物学]