商陆皂苷甲对兔滑膜细胞产生IL-1和TNF的影响  

Effects of esculentoside A on production of IL-1 and TNF by rabbit synovial cells

作　　者：郑钦岳[1] 王慧峰[1] 郑向民[1] 肖振宇[1] 易杨华 

机构地区：[1]第二军医大学药学院生化药学教研室,上海200433 [2]药学院天然药物化学教研室

出　　处：《第二军医大学学报》2001年第5期425-426,共2页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目 ( 3 9470 814 )

摘　　要：目的 :研究商陆皂苷甲 (Es A)对脂多糖 (L PS)刺激兔滑膜细胞产生白介素 1(IL - 1)和肿瘤坏死因子 (TNF)的影响。方法 :用胸腺细胞增殖法和用 L92 9细胞作靶细胞的生物测定法 ,分别检测与 Es A共育的受 L PS刺激的兔滑膜细胞培养上清中 IL- 1和 TNF的含量。结果 :Es A在 5～ 40 μg/ m l范围内能明显抑制 L PS诱导兔滑膜细胞产生 IL- 1和 TNF。结论 :Es A可抑制滑膜细胞产生 IL - 1和 TNF的作用提示其可能有助于消除类风湿性关节炎的关节炎症。Objective: To investigate the influence of esculentoside A(EsA) on production of IL 1 and TNF by rabbit synovial cells induced by LPS. Methods: levels of IL 1 and TNF in the supernatant of rabbit synovial cell were determined by examining proliferation of thymic cells and by bioassay L929 cells as target cells, respectively. Results: EsA in 5 40 μg/ml could significantly inhibit the production of IL 1 and TNF from rabbit synovial cells induced by LPS. Conclusion: EsA can inhibit the production of IL 1 and TNF from synovial cells. It suggests that EsA may play a role in improving the rheumatoid arthritis.

关 键 词：商陆皂苷甲 白细胞介素1 肿瘤坏死因子 兔 滑膜细胞 中药 

分 类 号：R285.5[医药卫生—中药学]