胎儿骨髓基质细胞与外源性细胞因子的协同造血支持作用  

Fetal bone marrow stromal cells support the in vitro expansion of human hematopoietic bone marrow progenitor cells: requirement for cell-to-cell interactions and colony-stimulating factors

作　　者：韩军[1] 王健民[1] 居小平[1] 徐激斌[2] 周虹[1] 闵碧荷[1] 

机构地区：[1]第二军医大学长海医院血液科,上海200433 [2]长海医院胸心外科

出　　处：《第二军医大学学报》2001年第5期439-442,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目 ( 3 980 0 0 5 5 )

摘　　要：目的 :探讨胎儿骨髓基质细胞与外源性细胞因子的协同造血支持作用。方法 :联合应用胎儿骨髓基质细胞与外源性细胞因子如重组人干细胞因子 (rh SCF)、IL - 3、IL - 6、粒巨噬细胞集落刺激因子 (GM- CSF)、粒细胞集落刺激因子 (G- CSF)、促红细胞生成素 (EPO)等先后对成人骨髓单个核细胞及 CD34 + 富集细胞培养 5 d至 2周。结果 :胎儿骨髓基质细胞与上述细胞因子具有良好的协同作用 ,CD34 +富集细胞以胎儿骨髓基质细胞 +SCF+IL- 3+IL- 6 +G- CSF+EPO培养 2周 ,可使细胞总数、粒 -单系造血祖细胞 (CFU- GM)、早期红系造血祖细胞 (BFU- E)及 CD34 +细胞分别扩增 (119.6± 30 .9)倍、(5 4.6± 17.4)倍、(2 5 .2± 4.4)倍、(11.1± 4.2 )倍。结论Objective: To elucidate the role of bone marrow stromal cells in cooperation with exogenous cytokines in hematopoiesis. Methods: Fetal bone marrow stromal cells (FBMSC) was combined with cytokines including SCF,IL 3,IL 6,GM CSF in a 5 day liquid culture system of adult bone marrow mononuclear cells, then we cultured bone marrow derived CD34 + enriched cells with FBMSC+SCF+IL 3+IL 6+G CSF+EPO for 2 weeks. Results:FBMSC were in good cooperation with above mentioned exogenous cytokines. When CD34 + enriched cells from adult bone marrow were cultured with combinations of FBMSC, SCF, IL 3, IL 6, G CSF and EPO, total nucleated cells, CFU GM, BFU E and CD34 + cells were increased by 119.6±30.9, 54.6±17.4, 25.2±4.4, 11.1±4.2 folds, respectively. Conclusion:FBMSC in cooperation with exogenous cytokines support the in vitro expansion of human hematopoietic progenitor cells efficiently.

关 键 词：胎儿骨髓细胞 骨髓移植 造血祖细胞 细胞因子 

分 类 号：R457.7[医药卫生—治疗学]