三种反义c-mycRNA对成纤维细胞NIH3T3的体外生物学效应  

BIOLOGICAL EFFECTS OF THREE KINDS OF ANTISENSE c myc RNA ON FIBROBLAST NIH3T3 in vitro

作　　者：曾嵘[1] 戴云[1] 安靓[1] 刘小蓉[1] 李进[1] 

机构地区：[1]第一军医大学组织学胚胎学教研室,广州510515

出　　处：《解剖学报》2001年第3期286-289,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 990 0 179)

摘　　要：目的　了解反义c myc基因稳定整合对正常细胞的影响。　方法　将分别载有c myc第 1、2和 3外显子的反义片段的 3种反义c myc重组逆转录病毒表达载体aM1、aM2和aM3,导入c myc正常表达的正常小鼠成纤维细胞系NIH3T3,并在基因稳定表达后进行各项检测。　结果　 3种反义载体导入明显抑制了NIH3T3c myc表达(抑制作用aM2 >aM3>aM1)、PCNA表达 (aM2 >aM1>aM3) ,使细胞体外生长增殖活性下降 (下降程度aM2 >aM1>aM3) ,aM2、aM3还使细胞周期出现了延长和DNA合成减少 ,并使NIH3T3软琼脂集落形成能力下降 (aM2 >aM3)。　结论　反义c mycRNA的导入对正常细胞的生长增殖能力有抑制作用 ,提示局部治疗和靶向治疗在反义cObjective In order to investigate the stable intergrative effects of antisense c myc on normal cells. Methods Three recombinant retroviral expression vectors, aM1, aM2 and aM3, which respectively loading reverse fragment of exon1, 2 and 3, were transferred into normal mouse fibroblast NIH3T3 in which normal c myc is expressed. Results Three antisense c myc significantly inhibited NIH3T3 c myc expression (aM2>aM3>aM1), PCNA expression (aM2>aM1>aM3), reduced cellular activity of growth and proliferation(aM2>aM1>aM3). aM2 and aM3 also could prolong cell cycle progress, reduced DNA synthesis and colony formation in soft agar(aM2>aM3). Conclusion Antisense c myc RNA inhibited the growth and proliferation of normal target cells, suggesting that local therapy or target therapy might be necessary for antisense c myc gene therapy. [

关 键 词：反义C-MYC 成纤维细胞 RNA NIH3T3 体外生物学效应 

分 类 号：R392.1[医药卫生—免疫学]