酰化纤溶酶原-链激酶(APSAC)的制备和生物学性质  

Preparation and Biological Properties of Acylated Human Plasm inogen-Streptokinase Activator Composite (APSAC)

作　　者：梁放[1] 孟庆繁[1] 滕利荣[1] 刘兰英[1] 孙宏[2] 陈维金[2] 关锋[2] 曲爱君[2] 刘淑英[2] 

机构地区：[1]吉林大学生命科学学院,长春130023 [2]长春生物制品研究所,长春130062

出　　处：《吉林大学自然科学学报》2001年第3期79-83,共5页Acta Scientiarum Naturalium Universitatis Jilinensis

摘　　要：建立在 L ys-Sepharose4B柱上用人纤溶酶原 ( HPg) ,链激酶 ( SK)和对氨基苯甲酸对脒基苯酚酯合成新型溶栓药物酰化纤溶酶原 -链激酶 ( APSAC)的一种新方法 ,该法使 APSAC的纯度和收率大为提高 ,分子量为 1 3 0 0 0 0 ,体外有明显溶栓效果 ,在兔体内半衰期 8.8h.用同方法制备的未经修饰的纤溶酶原 -链激酶复合物 ( PSAC)在兔血浆内半衰期仅为 1 2 min.Acylated human plasminogen streptokinase activator composite (APSAC) was prepared on the L lysine Sepharose 4B column with human plasminogen purified from human fresh plasma, purchased streptokinase and the chemical modification agent (4 amidinophenyl, 4'aminobenzioic acid). The HPLC showed that its M W is 130 000, and the fibrinolytic effect was proved by the fibrinolytic test in vitro. The unmodified plasminogen streptokinase activator composite (PSAC) prepared with the same method was cleared rapidly with an apparent half life of about 12 min in rabbit, but APSAC was removed more slowly ( t 1/2 8.8 h).

关 键 词：酰化纤溶酶原-链激酶复合物 制备 生物学性质 

分 类 号：Q556[生物学—生物化学]