普通念珠藻中水提、碱提多糖的分离、分析及活性研究  

ISOLATION, CHARACTERIZATION AND BIOLOGICAL ACTIVITIES OF POLYSACCHARIDES FROM NOSTOC COMMUNE BY WATER AND BAS

作　　者：盛家荣[1] 覃志英 陈超球[1] 

机构地区：[1]广西南宁师范学院 [2]广西南宁卫生防疫站

出　　处：《哈尔滨师范大学自然科学学报》2001年第2期80-84,共5页Natural Science Journal of Harbin Normal University

基　　金：广西青年基金资助 (桂科青 98110 18)

摘　　要：首次前后分别用蒸馏水、0 .5 mol Na OH溶液从普通念珠藻中提取、分离出水溶性多糖 SP和 JP,并对 SP、JP的一些简单理化性质进行了分析 ,完全酸水解后经高效液相色谱仪检测表明 ,SP含葡萄糖 ,JP含葡萄糖和木糖 ;将 SP、JP进行种子发芽活性试验 ,结果表明 :SP在 0 .5～ 2 mg/L范围内对实验物种子水稻、绿豆、大豆的发芽有明显的促进作用 ,发芽率分别比对照组增长9%、5 %、8% ;而 JP在 0 .5～ 40 mg/L范围内对实验物种子水稻、绿豆、大豆的发芽有明显的抑制作用 ,抑制率最高分别为 9%、9%、7% .对 SP、JP的作用机理进行了探讨 .Two water-soluble polysaccarides SP and JP were extracted and isolated from Nostoc commune by distilled water and 0.5 mol sodium hydroxide soluting resp., some characterization of SP and JP were tested and analysed. After hydrolysis and high performance liquid chromatography, SP was found wich made of one kind monosacharide, and it was glocose; JP was found wich made of two kinds monosacharides, and it was Glocose and Xylose. To make sure the effect of SP and JP on the growth of other species, the seeds of Oriza sativa, Phaseolus aureus, Glycine max and Brassia chinensis were used into the germination trial. Under the low concentration 0.5 mg/L^2 mg/L,SP raised the average germination rates of seeds of Oriza sativa, Phaseolus aureus, and Glycine max with 9%,5%,8%than the control. Under the concentration 0.5~40 mg/L,JP lowered the average germination rates of seeds of Oriza sativa, Phaseolus aureus, and Brassia chinensis with 9%,9%,7%than the control. The machanism of polysaccarides as plant-growth-regulater was analysed.

关 键 词：普通念珠藻 多糖 提取 分离 活性 

分 类 号：Q949.225[生物学—植物学]