普洛托品对兔胸主动脉平滑肌细胞增殖的影响  

Effects of protopine on proliferation of cultured rabbit aortic vascular smooth muscle cells

作　　者：邓敏[1] 宋秀媛[1] 王家富 姜波[3] 刘同美[1] 高尔[3] 丁怡[1] 郭方明[1] 

机构地区：[1]潍坊医学院病理生理教研室,261042 [2]泰安医学院病理生理教研室,271000 [3]潍坊医学院药理教研室,261042

出　　处：《中国药理学通报》2001年第3期306-309,共4页Chinese Pharmacological Bulletin

基　　金：山东省自然科学资金!资助课题 ;NoQ95 0 0 42 0 ;P山东省卫生厅立项课题!No 1998CA1CCB2

摘　　要：目的　观察普洛托品 (Pro)对正常和内皮素 (ET)诱导的血管平滑肌细胞 (VSMC)增殖的影响。方法　采用组织块贴壁法培养兔胸主动脉VSMC ,应用细胞计数、[3 H] TdR参入量、微量MTT比色测定及电镜法 ,观察Pro对VSMC增殖、DNA合成和细胞超微结构的变化。结果　ET对VSMC的增殖和DNA合成有显著的促进作用 ;1、10、10 0μmol·L-1浓度Pro能够逆转ET所致的 [3 H] TdR参入量、MTT比色的吸光度、细胞计数的增多及电镜下细胞超微结构的改变 ,且随着浓度的增加及时间的延长 ,抑制作用愈显著 ;10、10 0 μmol·L-1浓度Pro对未经ET处理的VSMC有抑制其增殖作用 ,但其抑制作用弱于ET处理组。结论　Pro可显著地抑制正常及ET诱导的VSMC增殖 ,并且对ET诱导的VSMC增殖抑制作用更明显 ,提示Pro可作为临床防治动脉粥样硬化的有效药物。AIM To study the effect of protopine (Pro) on proliferation of cultured rabbit aortic vascular smooth muscle cells (VSMC) induced by endothelin 1(ET) or without ET. METHODS The method of celluar culture, the number of VSMC, [ 3H] TdR incorporation into DNA assay, MTT method and electronic microscopy were performed for the effect of Pro on proliferation of cultured rat vascular smooth muscle cell. RESULTS It was observed that exposure of VSMC to ET could markedly stimulate the proliferation of VSMC and increased respectively in MTT OD values and the DNA synthesis of VSMC. The concentration 1 μmol·L -1 ,10 μmol·L -1 , 100 μmol·L -1 of Pro significantly decreased the number of VSMC, the absorbance (A) of MTT and [ 3H] TdR incorporation induced by ET 1( P <0 01 or P <0 05). While the proliferation of normal VSMC was decreased only by the concentration 10 μmol·L -1 , 100 μmol·L -1 of Pro, and the inhibition rate is lower than ET groups. Furthermore, Pro could antagonize morphological changes of VSMC. CONCLUSION Pro has an effective inhibition on VSMC proliferation induced by ET 1 or without ET 1, and the inhibition rate increased by Pro concentration rising and time passing by. Furthermore, the inhibition of Pro to the proliferation of VSMC is more significantly induced by ET than treated without ET. This suggests that Pro could be of interest to prevent and cure arteriosclerosis clinically.

关 键 词：普洛托品 内皮素 血管平滑肌细胞 细胞增殖 

分 类 号：R543.5[医药卫生—心血管疾病] R972[医药卫生—内科学]