前列腺素E_1对豚鼠心室肌细胞ATP敏感K^+通道的影响  

Effect of prostaglandine E_1 on ATP-sensitive K^+ channel in ventricular myocytes of guinea pig

作　　者：杨光[1] 张沈丽[2] 胡永校[3] 肖德绵[4] 李金鸣[2] 

机构地区：[1]中国医科大学附属第一临床医院循环内科,沈阳110001 [2]中国医科大学附属第一临床医院基础医学院药理学教研室,沈阳110001 [3]中国医科大学附属第一临床医院胸外科,沈阳110001 [4]中国医科大学附属第一临床医院心脏外科,沈阳110001

出　　处：《中国药理学通报》2001年第3期326-328,共3页Chinese Pharmacological Bulletin

基　　金：辽宁省自然科学基金!资助项目 ;No 6190 73

摘　　要：目的　从离子通道水平 ,探讨前列腺素E1(PGE1)对ATP敏感K+ (KATP)通道的作用。方法　膜片钳制技术全细胞记录模式。结果　PGE1可诱导KATP通道开放并呈浓度依赖关系。保持电位 - 40mV ,指令电位 +2 0mV ,持续时间 1s条件下 ,10 μmol·L-1PGE1使外向K+ 电流由给药前的 (2 2 7± 0 34 )nA增加到 (5 46± 0 34 )nA(n =6 ,P <0 0 1) ,增加了 (3 18± 0 2 3)nA。并且增加的钾电流可被KATP通道特异阻断剂Glibenclamide(10 μmol·L-1)抑制 ,抑制率是 6 3%± 7% (n =5 ,P <0 0 1)。AIM To investigate the effect of prostaglandin E 1(PGE 1) on ATP sensitive K + (K ATP ) channel in ventricular myocytes of guinea pig and explain the physiological , pathophysiological and pharmacological mechanism of PGE 1 on channel level. METHODS Patch clamp technique with standard whole cell configuration. Holding potential was -40 mV, commanding potential was -100 mV～+60 mV, and duration was 1 s. RESULTS PGE 1 induced K ATP channel opening in a concentration dependent manner. When holding potential was -40 mV and command potential was +20 mV, K + current increased from (2 27±0 34) nA to (5 46±0 34) nA( n=6, P <0 01) after perfusion with external solution containing 10 μmol·L -1 PGE 1. The increased K + current could inhibit by specific K ATP channel inhibitor glibenclamide (10 μmol·L -1 ), and inhibitory rate was 63%±7%( n=6,P <0 01). CONCLUSION PGE 1 opens K ATP channel in ventricular myocyte of guinea pig.

关 键 词：前列腺素E1 ATP敏感性K^+通道 心室肌细胞 膜片钳制技术 钾通道 

分 类 号：R977.6[医药卫生—药品]