转化生长因子β1对大鼠系膜细胞醛糖还原酶表达的影响  

Effect of aldose reductase expression by transforming growth factor β1 on rat mesangial cell

作　　者：林文生[1] 张农[1] 秦蓉[1] 刘琛[1] 郭慕依[1] 

机构地区：[1]复旦大学医学院病理学教研室,上海200032

出　　处：《中华医学杂志》2001年第12期744-747,共4页National Medical Journal of China

基　　金：上海市教委重点学科基金资助项目 (B990 80 2 ) ;复旦大学中华医学基金会基金资助项目 (93 5 83 )

摘　　要：目的　对转染人转化生长因子 (TGF) β1基因的大鼠系膜细胞 (MsC)进行相关反应性基因的筛选 ,并观察TGF β1对其中之一的醛糖还原酶表达的影响。 方法　采用SSH PCR和反向杂交法对转染人TGF β1基因的MsC筛选其相关反应性基因 ,并进行测序及与GenBank资料作同源性序列比较 ;又分别应用半定量RT PCR、Westernblot法观察其在动物体内和培养的MsC中表达的变化。结果　经筛选获得的 2 8个反应性基因cDNA片段 ,1个长度为 337bp的cDNA片段与已知醛糖还原酶同源性完全一致 ;体外培养的MsC经TGF β1作用 ,该酶mRNA和蛋白表达增强 ;在大鼠抗Thy1系膜增生性肾小球肾炎病变肾组织中 ,该酶mRNA和蛋白的表达随TGF β1表达上调而明显增强。结论　醛糖还原酶是一个与TGF β1基因表达密切相关的多元醇代谢通路的关键酶 ,可能参与TGFObjective To screen the responsive genes related to transforming growth factor β1(TGF β1)in rat mesangial cell (MsC) transfected by human TGF β1 gene, using suppression subtractive hybridization PCR(SSH PCR); then to detect the expression of aldose reductase gene on cultured rat MsC and renal cortex of rats with anti Thy1 glomerulonephritis. Methods SSH PCR technique and differential screening for detecting the responsive genes related to TGF β1; Semi quantification RT PCR and Western blot analysis for identifying the expression of aldose reductase on MsC in vitro and in vivo. Results Compared with normal MsC, it was proved that 28 responsive genes related to TGF β1 in MTG1. There was an expression sequence tag (EST), approximate 337 bp segment, completely identical to the data of aldose reductase gene from GenBank. Elevated levels of aldose reductase mRNA and protein were found on cultured rat MsC treated with TGF β1 and renal cortex of rat with anti Thy1 glomerulonephritis. Conclusion Aldose reductase, rate limiting enzyme in the polyol pathway, was closely related to TGF β1 expression. It is possible that upregulation of aldose reductase expression may be involved in the development of glomerulosclerosis mediated by TGF β1.

关 键 词：转化生长因子Β1 醛还原酶 肾小球肾炎 

分 类 号：R692[医药卫生—泌尿科学]