免疫荧光双标记技术检测胎盘HBsAg-抗-HBs复合物  

Detection of HBsAg-anti-HBs complex by double-labelling immunofluorescence method

作　　者：王歆[1] 徐德忠[1] 刘蓬勃[1] 李玉松[2] 王素萍[1] 

机构地区：[1]第四军医大学预防医学系流行病学教研室,陕西西安710033 [2]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2001年第12期1125-1128,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 !(3 980 0 15 2 )

摘　　要：目的　探讨乙肝病毒 (HBV)以何种形式感染胎盘滋养层细胞 .方法　通过免疫荧光双标记技术检测 12例血清HBs Ag阳性母亲的胎盘滋养层细胞 HBs Ag-抗 - HBs复合物 .结果　血清学 HBs Ag阳性母亲胎盘滋养层细胞中存在 HB-s Ag-抗 - HBs复合物 .结论　提示 HBV可能通过 HBs Ag与抗 - HBs结合成复合物的方式进入胞内 ,导致胎盘屏障的第一层细胞受到AIM To find out which form Hepatitis B Virus (HBV) takes in the infection of the first layer cell of placenta barrier trophoblast cells. METHODS HBsAg anti HBs complexes in HBsAg seropositive maternal placenta tissues were tested by double labeling immunofluorenscence method visualized with laser scanning confocal microscope. RESULTS HBsAg anti HBs complexes were found mainly on trophoblast cells (TC) of HBsAg seropositive maternal placenta tissues. CONCLUSION HBsAg and anti HBs are formed as HBsAg anti HBs complexes before entry into trophoblast cells, which may result in the infection of the first layer cell of placenta barrier.

关 键 词：乙型肝炎病毒 胎盘 抗原抗体复合物 免疫荧光双标记 HBsAg-抗-HBs复合物 

分 类 号：R512.62[医药卫生—内科学] R714.56[医药卫生—临床医学]