大鼠心室成纤维细胞培养液对自身细胞增殖的作用  

Role of ventricular fibroblasts in promotion proliferation of fibroblasts in neonatal rats

作　　者：郭晓霞[1] 徐洪涛[2] 龚素珍[3] 

机构地区：[1]北京职工医学院临床医学系,北京100036 [2]海军总医院心血管内科,北京100003 [3]广州医学院神经所,广州510182

出　　处：《基础医学与临床》2001年第3期268-271,共4页Basic and Clinical Medicine

摘　　要：利用成纤维细胞培养、同位素掺入测定和RT PCR方法 ,探讨心室成纤维细胞条件培养液促进成纤维细胞自身增殖作用。心室成纤维细胞条件培养液能明显增加细胞 [3H] 胸腺嘧啶核苷的掺入率 ,并具有剂量依赖性 ;促进细胞自身的c fos基因的表达 ,刺激后 1h达高峰。ETA 受体拮抗剂BQ12 3 能部分阻断条件培养液增加成纤维细胞增殖作用 ,而AT1受体拮抗剂CV11974和α 肾上腺素受体拮抗剂regitin无此效果。提示心室成纤维细胞具有自分泌功能 ,能分泌内皮素等生物活性物质 。We studied the proliferation on the ventricular fibroblasts by the incorporation rate of [ 3H]TdR and the expression abundance of c fos gene The results showed that FCGM could significantly increase the [ 3H]TdR incorporation rate of fibroblasts , and had dose dependent effect, FCGM also promoted the c fos gene expression of fibroblasts, and the expression abundance was the highest after stimulated for 1 hour BQ 123 ,an ET A receptor antagonist could partially block the effect of FCGM on promotion proliferation of ventricular fibroblasts, but CV11974,AT 1 receptor antagonist and regitin, α adrenergic receptor didnt The results suggested that the ventricular fibroblasts had autocrine effect,and secreted endothelin and other bioactive substances,they could promote the proliferation of fibroblasts

关 键 词：成纤维细胞 [^3H]-胸腺嘧啶核苷 C-FOS MRNA 心脏 细胞增殖 

分 类 号：R331.3[医药卫生—人体生理学]