TT病毒在恒河猴实验感染的组织嗜性  

Tissue tropism of TT virus in experimentally infected Rhesus monkey

作　　者：肖红[1] 祁学忠[1] 文维群[1] 王燕军[1] 梁蔚芳[1] 骆抗先[1] 

机构地区：[1]第一军医大学附属南方医院感染内科,广州510515

出　　处：《中华传染病杂志》2001年第3期159-161,共3页Chinese Journal of Infectious Diseases

摘　　要：目的　了解TT病毒 (TTV)的组织嗜性。方法　从 5只实验感染病毒的恒河猴各组织中抽提DNA ,分别用病毒双链探针或单链负义探针 ,与吸附在纤维膜上的组织DNA进行斑点杂交。结果　双链探针与肝、骨髓、脾、胃、小肠、血清和大肠杂交阳性 ,表明这些组织中都有TT病毒的存在。单链负义探针与各组织杂交 ,仅肝、骨髓和小肠为阳性 ,提示可能存在作为病毒复制中间体的正义单链DNA。结论　肝、骨髓和小肠可能是病毒的复制场所 ,故TT病毒可能具嗜肝性 。Objective To study tissue tropism of TT virus in experimentally infected Rhesus monkey. Methods Various tissues were collected from 5 experimentally infected monkeys during the viremic period. Total DNA was extracted from tissues of 5 experimentally infected Rhesus monkeys. A dot hybridization was done with virus double DNA strand probe or single antisense strand probe. Results The double strand probe was hybridized with DNA of liver, bone marrow, spleen, stomach, small intestine and colon. The single strand antisense probe was hybridized only with DNA of liver, small intestine and bone marrow of all 5 monkeys, but not with that of other tissues.Conclusions As the viral genome is of negative polarity, the plus stranded fragment identified in our study might be a replicative intermediate, and is only demonstrated in liver, small intestine, and bone marrow by dot blot hybridization with single stranded antisense probe. It suggests that TT virus might be, tropism of liver, small intestine, and bone marrow, and replicate in tissue mentioned.

关 键 词：TT病毒 恒河猴 实验感染 组织嗜性 输血传播病毒 

分 类 号：R512.6[医药卫生—内科学]