远志抽提物DX-1对神经母细胞瘤分化的诱导作用  

Study on telomerase activity during induction of axon-like and dendrite-like processes in neuroblastoma cells.

作　　者：张萌[1] 文剑明[1] 李文魁[2] 麦乃歧[2] 

机构地区：[1]中山医科大学病理学教研室,广州510080 [2]香港浸会大学生物系

出　　处：《中国神经精神疾病杂志》2001年第4期279-281,I001,共4页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的 观察神经母细胞瘤细胞分化诱导后形态学改变和端粒酶活性的变化。方法 用中药远志抽提物1,7-二羟夹氧蒽酮（DX-1）诱导神经母细胞系Neuro-2A细胞分化,观察分化后瘤细胞的存活率、细胞倍增时间,以及细胞轴突树状突形成、长短和数目,并用TRAP银染法检测分化前后瘤细胞端粒酶活性的改变。结果 用DX-1处理后,Neuro-2A细胞生长受明显抑制,其倍增时间延长1.1-13.0倍。在高剂量组（0.1mmol/L）大部分细胞死亡,仅存少量细胞分化成熟。各处理组的瘤细胞呈现神经细胞分化,胞浆具有轴突和树状突样突起,一些突起互相对接,或交织成网状结构。然而,经中、低剂量DX-1作用后,瘤细胞端粒酶活性未见明显的差异,仅在高剂量时端粒酶活性明显下降,甚至完全消失。结论DX-1可诱导体外培养的Neuro-2A细胞分化。在中、低剂量时,瘤细胞出现轴突和树状突突起。在高剂量时,DX-1对瘤细胞有一定的抑制和杀灭作用。在瘤细胞分化过程中,端粒酶活性并不出现明显的变化。Objective To observe telomerase activity change during differentiation induction of neuroblastoma cells. Methods Differentiation of neurobalstoma cells was induced by 1,7-dihydroxanthone (DX-1) extracted from polygala caudata, doubling time, length and number of axon-like and dendrite-like processes induced were observed during the differentiation of the cells. Telomerase activity was detected by TRAP-silver staining method. Results After treatment with DX-1, proliferation of neuroblastoma cells was inhibited significantly. The population doubling time of the cells prolonged 1.1 - 13.0 times. Most of cells died but some differentiated into mature neuron-like cells in high dose of DX-1 treatment. Exposure to DX-1 induced axon-like and dentrite-like process outgrowth along with cell body which crossed as a net or contacted end to end. However, telomerase activity did not changed markedly in the cells after treated with middle or low dose of DX-1. Only in high dose group telomerase activity decreased and eventually disappeared. Conclusions DX-1 may induce in vitro differentiation of neuroblastoma cells and give rise to neuriles with axon-like and dendrite-like characteristics in the cells. High dose of DX-1 inhibits proliferation and affects survival of neuroblastoma cells. Telomerase activity of the cells, however, does not change remarkably during the differentiation of the cells.

关 键 词：神经母细胞瘤 神经突起 分化 端粒酶活性 DX-1 

分 类 号：R273[医药卫生—中西医结合]