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机构地区:[1]内蒙古民族大学动物科技学院,内蒙古通辽028042 [2]中山大学生命科学学院,广东广州510275 [3]辽宁省辽阳县鱼种场,辽宁辽阳111218
出 处:《中国水产科学》2001年第2期48-51,共4页Journal of Fishery Sciences of China
基 金:国家自然科学基金资助项目 (39970 5 86 ) ;内蒙古自然科学基金资助项目 (96 10E45 )
摘 要::以珠江和辽河流域的鲇 (Silurusasotus)为研究对象 ,在室内进行了不同组合的激素和药物注射实验。结果表明 ,注射促黄体素释放激素类似物 (LHRH A)和多巴胺拮抗物地欧酮 (DOM )药物组合 (LHRH A+DOM ) ,剂量为 ( 10 μg +5mg) /kg鱼体重 ,能快速而大量地诱导鲇血清促性腺激素 (GtH)水平的升高 ,进而诱导产卵 ,产卵率为 90 % ,显著高于其他实验组 ;采用LHRH A +DOM药物组合对 2 92 6组鲇进行了人工催产 ,注射剂量为 ( 7μg +5mg) /kg鱼体重 ,平均催产率为 70 % ,最高 80 % ,获得鱼苗 2 0 7万尾。The catfish, Silurus asotus, were captured from Pearl River(June~July, 1999) and Liaohe River (May~June, 2000). In the ovulation induction experiment, 5 treatments were designed, which were injected with P.S (control), LHRH A, LHRH A+DOM, common carp PG+LHRH A, and common carp PG+HCG, respectively. The results showed that in the treatment with a dose of LHRH A+DOM (10 μg+5 mg)/kg body weight, the induction led to a large secretion of GtH to a high level in the catfish, furthermore resulted in the spawning with a spawing rate of 90% which was obviously higher than the other treatments. In the artificial breeding experiment, 2 926 individuals were injected with LHRH A+DOM at a dose of (7 μg+5 mg)/kg body weight, getting an average spawning rate of 70%, the highest 80%, and 2.07 million larvae were hatched.
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