鳗弧菌口服微胶囊疫苗的制备及其对鲈鱼的免疫效果  

Oral vaccination of sea perch, Lateolabrax japonicus, against Vibrio anguillarum using microencapsulated vaccine

作　　者：余俊红[1] 沈继红[2] 王祥红[1] 王宝坤[1] 纪伟尚[1] 胡应劭[3] 徐怀恕[1] 

机构地区：[1]青岛海洋大学生命学院,山东青岛266003 [2]国家海洋局第一海洋研究所,山东青岛266003 [3]香港中文大学生物系

出　　处：《中国水产科学》2001年第2期76-79,共4页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目 (39870 5 81) ;国家"九七三"资助项目 (G1999 0 12 0 0 4)

摘　　要：以分离自患病鲈鱼 (Lateolabraxjaponicus)的致病菌W - 1为材料 ,利用喷雾微胶囊技术制备口服微胶囊疫苗。结果表明 ,喷雾干燥条件对疫苗微胶囊颗粒影响较大 ;其适宜条件为 :进料温度 4 0℃ ,进料速度 4 0ml/min ,热风温度 2 0 0℃ ;其制备的微胶囊疫苗平均粒径为 4 5.5μm ,平均含菌量为 2 .79× 10 9mg- 1干粉。将此微胶囊疫苗及全细胞疫苗直接拌入饵料口服免疫接种鲈鱼幼鱼 ,1周后以W - 1活菌攻毒 ( 2 .5×10 6CFU /尾 )。结果表明 ,微胶囊疫苗组的 1周累积死亡率 ( 4 1.7% )低于全细胞疫苗组 ( 4 5.0 % ) ,均低于对照组 ( 95.0 % )。 2个免疫组都有一定的免疫保护力 ,微胶囊疫苗组和全细胞疫苗组的免疫保护力分别为56.1%和 52 .6%。The oral microencapsulated vaccine was prepared using Vibrio anguillarum, isolated from diseased sea perch, Lateolabrax japonicus while a technique of spray drying microencapsulation was employed. The optimum conditions for spray drying were:feed temperature 40℃, feed speed 40 ml/min, hot wind temperature 200℃. The microencapsulated vaccine particles produced had an average diameter of 45.5 μm and the average bacterial mass of 2.79×10 9 mg -1 particle powder. Then, the sea perch were fed directly the diets mixed with the microencapsulated vaccine and the whole cell vaccine, respectively, for 5 d, while the control only the normal diet. One week after that all the experimental fish were injected with V. anguillarum and the 1 week observation results showed that the 2 vaccine fed groups were much lower than control in 1 week cumulative mortality, which were 41.7%(microencap sulated group), 45.0%(whole cell group) and 95% (control), respectively. The relative percentage survival of the 2 vaccine fed groups were 56.1% and 52.6%, respectively.

关 键 词：鲲弧菌 口服微胶囊疫苗 制备 鲈 鱼 免疫保护力 弧菌病 

分 类 号：S942.5[农业科学—水产养殖] S941.42[农业科学—水产科学]