球壳孢目真菌同工酶电泳研究 I.分类学意义的评价  

Studies on the Isozymes Electrophoresis of Sphaeropsidales Species

作　　者：刘伟成[1] 吕国忠[2] 周永力[3] 梁景颐[2] 孙军德[2] 白金铠[2] 

机构地区：[1]解放军军需大学农副业生产系,吉林长春130062 [2]沈阳农业大学,辽宁沈阳110161 [3]中国农业科学院作物所,北京100081

出　　处：《沈阳农业大学学报》2001年第2期102-106,共5页Journal of Shenyang Agricultural University

摘　　要：对球壳孢目5属10种17个菌株进行了菌体可溶性蛋白、酯酶同工酶和过氧化物酶同工酶聚丙烯酰胺凝胶电泳分析 ,不同培养时间样品的电泳图谱显示菌体培养时间的长短对实验结果有一定的影响 ,球壳孢目真菌以培养5～10d为宜 ;不同种的菌株的图谱差异显著 ,而种内不同菌株间的谱带一致性较高 ,表明可溶性蛋白和同工酶电泳图谱对球壳孢目真菌种级水平的分类具有重要作用 。The soluble mycoprotein and isozyme of 17 isolates from 10 species belonging to 5 genera were analysed with polyacrylamide gel electrophoresis. The marked difference of protein patterns and zymograms between different species and the considerable identity between isozymes from the same species indicated that the electrophoretic analysis of protein and isozyme, especially esterase isozyme, was a useful supplementary means for the classification of sphaeropsidales at the level of species. Different patterns of samples from the same isozyme cultured in different days could be produced, so the identical experiment conditions were essential for isozyme analysis.

关 键 词：球壳孢目 分类学意义 真菌 同工酶电泳 可溶性蛋白 

分 类 号：Q949.331.1[生物学—植物学]