枸杞脱毒苗的诱导及光合特性的分析研究  

INDUCTION OF VIRUS-FREE LYCIUM BARBARUM L. AND REC HSEACH ON PHOTOSYNTHESIS OF PLANTS

作　　者：曹有龙[1] 唐琳[1] 颜钫[1] 罗青[2] 郭宏玲 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]宁夏农林科学院,银川750001 [3]宁夏林业厅,银川750001

出　　处：《四川大学学报（自然科学版）》2001年第4期550-553,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：利用茎尖分生组织 (0 .2～ 0 .4mm)培养和花药培养 ,获得了枸杞脱毒苗 .茎尖培养脱毒率在 72 .7%～ 87.5 %之间 ,花药培养脱毒率在 70 .0 %～ 76 .5 %间 .进行 38℃下热处理茎尖分生组织组培苗 ,可提高脱毒效果 .叶绿素含量和光合强度测定表明 ,脱毒苗与对照相比 ,叶绿素含量增加 2 .3～ 2 6 .9% ,光合强度增加 6 .9～ 12 .1%Virus free Lycium barbarum L. Were obtained by runner tip meristem culture and anther culture, and the frequency of virus free plants were 72.7%～95.0% and 89.7%～98.2%respectively. Heat treatment of plantlets from meristem culture at 38℃ increased the frequency of virus free plants, compard with the controls, the virus free Lycium barbarum L. increased 2.3%～26.9% in concentration of chorophyl and 6.9%～12.1%photosynthesis rate.

关 键 词：枸杞 脱病毒苗 组织培养 光合作用 药用植物 花药培养 叶绿素 

分 类 号：S567[农业科学—中草药栽培] Q945.11[农业科学—作物学]