荧光假单胞菌株M18防治甜瓜蔓枯病害  

Biocontroling of Melon Gummy Stem Blight (Mycosphaeralla melonis) Using Pseudomonas fluorecens M18

作　　者：祝新德[1] 冯镇泰 许煜泉[1] 张霞[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]上海农乐生物制品股份有限公司,上海201600

出　　处：《上海交通大学学报》2001年第7期1062-1065,共4页Journal of Shanghai Jiaotong University

基　　金：国家自然科学基金资助项目 (30 0 70 395 );上海市科委科技发展基金资助项目 (995 4130 2 8)

摘　　要：荧光假单胞菌株 M1 8在离体条件下 ,能有效抑制甜瓜蔓枯病菌的菌丝生长 .经甜瓜蔓枯病菌的苗期接种试验 ,M1 8的菌悬液 (1 0 8CFU/m L )对甜瓜蔓枯病的防治效果达到 80 %左右 .在苗期接种抗性诱导试验中 ,发现荧光假单胞菌株 M1 8还具有诱导甜瓜幼苗产生系统性抗甜瓜蔓枯病菌感染的能力 .从 M1 8培养液中 ,提取并纯化了抗生物质吩嗪 - 1 -羧酸 (PCA) ,该物质对甜瓜蔓枯病菌具有强力抑菌作用 ,EC50 值仅为 3.2 4 μg/m L.在大田试验中 ,M1 8可以保持 80 %的防治效果 ,显著提高甜瓜产量 ,并且还发现在蔓枯病发病初期 ,M1Pseudomonas fluorecens M18 can efficiently inhibit the mycelium growth of melon gummy stem blight ( Mycosphaeralla melonis) in vitro. The controlling efficiency of M18 suspension (10 8 CFU/mL) can reach to about 80% under the condition of both seedling inoculation experiments and field performance. It was also found that P.fluorecens M18 could induce the melon seedling to produce a systemic resistance against M.melonis . An antifugal substances phenazine 1 carboxylic acid (PCA) was extracted and purified from P.fluorecens M18 and the results from bioassay experiments show that this substance has strong antifugal activity against M.melonis and the EC 50 value was 3.24 μg/mL only.

关 键 词：生物防治 甜瓜蔓枯病 荧光假单胞菌 吩嗪-1-羧酸 M18 抑菌作用 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治] S476.11[农业科学—植物保护]