以酵母双杂交系统筛选与糖基转移酶相互作用的蛋白质  

Screen for Proteins That Can Interact with Glycosyltransferases with Yeast Two-Hybrid System

作　　者：蒋能群[1] 张颂文[2] 张惟杰[1] 顾建新[2] 

机构地区：[1]上海交通大学生命科学技术学院,上海200030 [2]上海医科大学基础研究中心,上海200032

出　　处：《上海交通大学学报》2001年第7期1076-1080,共5页Journal of Shanghai Jiaotong University

摘　　要：用 N -乙酰氨基葡萄糖基转移酶 (GNT )和β 1 ,4-半乳糖基转移酶 (GT )作诱铒 ,以酵母双杂交系统分别从胎肝 c DNA文库中筛选到一种能与 GNT 发生相互作用的蛋白质 ,筛选到两种能与 GNT 发生相互作用的蛋白质 .与 GNT 作用的是早幼粒细胞白血病锌指蛋白(PLZF) ,属于视黄酸受体家族的一种转录因子 .提出了一种新的相互作用假设模型 :PLZF是GNT 的底物之一 .另发现纤粘蛋白 (FN)与 GT 作用 ,并推测此相互作用参与了细胞粘附 .以上两种相互作用都是已知蛋白间的未知相互作用 .还发现 GT 与由新基因编码的蛋白相互作用 .First part, N acetylglucosaminyltransferase Ⅲ(GNTⅢ) was used as the bait, and then from human fetal liver cDNA library, a protein which can interacts with GNTⅢ were obtained with yeast two hybrid system. Promyelocytic leukaemia zinc finger protein (PLZF) is a transcriptional factor and a member of retinoic acid receptor family. We speculate that PLZF is one of the subjects of enzyme GNT Ⅲ. Second part, β 1,4 galactosyltransferase Ⅱ(GTⅡ) was used as the bait, and then from human fetal liver cDNA library, two proteins which can interact with GTⅡ were obtained with yeast two hybrid system. One of the two proteins is fibronectin, and this interaction may take part in intercellular conglutination. The other is a protein coded by a new gene.

关 键 词：N-乙酰氨基葡萄糖基转移酶Ⅲ β1 4-半乳糖基转移酶Ⅱ 酵母双杂交系统 相互作用 蛋白质 

分 类 号：Q51[生物学—生物化学]