IGF-1对急性肾功衰竭的治疗作用研究  

THE THERAPEUTIC EFFECT OF INSULIN-LIKE GROWTH FACTOR-I IN ACUTE RENAL FAILURE

作　　者：马行一[1] 杨立川[1] 冀玲[1] 樊均明[1] 刘芳[1] 李献[1] 钟慧[1] 

机构地区：[1]四川大学华西附一院,四川成都610041

出　　处：《华西药学杂志》2001年第3期171-174,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川省卫生厅科研基金资助(962007);四川省科委科研基金资助

摘　　要：目的：探讨胰岛素样生长因子Ｉ（ＩＧＦ－Ｉ）对急性肾功能衰竭（ＡＲＦ）的治疗作用。方法：建立大鼠缺血性急性肾衰竭模型，Ｗｉｓｔａｒ大鼠４８只随机分成三组：假手术对照组（ｎ＝１６），ＩＡＲＦ组３２只夹闭双肾动脉４５ｍｉｎ建立ＩＡＲＦ模型（ｎ＝３２），其中随机分１６只为ＩＧＦ－Ｉ组：每日皮下注射ｒｈＩＧＦ－Ｉ一次（２５０ｎｇ．ｋｇ－１）。观察内生肌酐清除率（Ｃｃｒ），２４ｈ尿蛋白，β２－ＭＧ，血、尿 ＩＧＦ－Ｉ，血栓素Ｂ２（ＴＸＢ２），６－酮－前列腺素 Ｆ１（６－ｋｅｔｏ－ＰＣＦ１α）水平及肾脏组织形态学改变。结果：缺血后ＩＡＲＦ组Ｃｃｒ显著下降，２４ｈ尿蛋白，β２－ＭＧ下降，血、尿ＴＸＢ２及肾重／体重增高，６－Ｋｅｔｏ－ＰＧＦ１及尿 ＩＧＦ－１水平降低，与对照组相比差异显著。 ＩＧＦ－Ｉ组 Ｃｃｒ升高，且较快恢复到对照水平；２４ ｈ尿蛋白，β２－ＭＧ及血、尿ＴＸＢ２下降，６－ｋｅｔｏ－ＰＧＦ１及尿ＩＧＦ－Ｉ水平增高；肾组织学观察肾小管损伤程度减轻，肾小管上皮再生恢复快。结论：ＩＧＦ－Ｉ能改善 ＩＡＲＦ肾功能，减轻肾小管损伤程度，促进肾小管上皮再生恢复。OBJECTIVE: To explore the mechanism of in- sulin-like growth factor-I(IGF-1) in acute renal failure(ARF). METHOD: Wistar rats were divided into three groups:Pseuoop- erative group (control, n = 16); IARF group(n = 32): rats were subjected to 45 minutes of bilateral renal ischemia; IGF-1 group(n = 16 ): a subcutaneous injection of rhIGF-I (250ng· Kg-1) was given per day after releasing clamped bilateral renal artery, Creatinine clearance(Ccr), urine protein Per 24 hours, serum and urine β2-microglobulin(β2-MG), IGF-1, thrombox- ane(TXB2), prostaglandin F1alpha (PGF1) were measured and rend histology was observed. Ccr concentration in IARF group decreased obviously, while proteinuria per 24 hoars, β2-MG, kidney weight/body weight, serum and urine levels of TXB2 were higher and serum and urine levels of PGF1α and urine IGF were lower than those in the control group. RESULTS: To compared with IARF group, Ccr concentration showed increase and a more rapid return toward hasal values, the serum and urine levels of TXB2, urine protein per 24 hours, β2-Mg reduced and serum and urine levels of PGF1α and urine IGF increased in IFG-I group. Kidneys from rats that had received IGF-I were improved in histopathological apperance. CONCLUSION: IGF-I improves renal function, hastens recovery and accelearates re- generation of damaged tubular epithelia following ischernic acute renal failure.

关 键 词：胰岛素样生长因子-Ⅰ 急性肾功能衰竭 治疗 

分 类 号：R692.5[医药卫生—泌尿科学]