电针调控大鼠胃酸分泌的机制  

of Health Statistics, Faculty of Preventive Medi cine, 5Department of Electrecopy, Faculty of Preclinical Medicine, Fourth Military Medical Univer sity

作　　者：赵保民[1] 黄裕新[1] 赵宁侠[2] 刘梅[3] 尚磊[4] 黄梅[5] 王庆莉[1] 

机构地区：[1]第四军医大学唐都医院消化科,陕西西安710038 [2]第四军医大学唐都医院中医科,陕西西安710038 [3]第四军医大学唐都医院药剂科,陕西西安710038 [4]第四军医大学预防医学系卫生统计学教研室 [5]第四军医大学基础部电镜中心

出　　处：《第四军医大学学报》2001年第9期786-789,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目! (39970 888)

摘　　要：目的　探讨电针足三里穴对大鼠胃酸分泌的调控机制 .方法　 75只 SD大鼠随机分为 5组 ,在清醒状态下针刺双侧足三里穴 ,以空白对照、非经非穴作对照 ,三阴交穴作经穴对照 .同步测定胃液量、p H值及胃液酸度 ,放免法测定血浆、胃液、胃粘膜胃泌素 (Gas)和表皮生长因子 (EGF)含量 ,透射电镜观察胃粘膜腺细胞超微结构 .结果　电针刺激足三里穴后大鼠空腹胃液量明显减少 [(0 .30± 0 .11) vs(0 .6 3± 0 .12 )m L,P<0 .0 1],胃液酸度明显降低 [(30± 3) vs(4 1± 1)mmol· L- 1 ,P<0 .0 5 ],胃液 p H升高 .胃粘膜 Gas含量增多[(136± 17) vs(73± 4) ng· L- 1 ,P<0 .0 1],血浆 Gas含量下降 [(84± 2 2 ) vs (10 8± 30 ) ng· g- 1 ,P<0 .0 1],胃液 Gas无变化 ;胃液及胃粘膜 EGF含量显著升高 [(3.2± 1.1) vs(1.8± 0 .4) μg· L- 1和 (5 .1± 0 .8) vs(2 .4± 0 .4) μg· g- 1 ,P<0 .0 1],血浆 EGF无变化 .胃壁细胞泌酸小管不扩张 ,小管泡充盈不足 ,且数量不减少 ,非经非穴组及空白对照组各项指标无显著变化 .结论　电针足三里穴抑制大鼠胃 G细胞Gas释放 ,降低血浆 Gas,增加胃粘膜、胃液 EGF含量 ;电针足三里可改变胃壁细胞超微结构 ,抑制胃壁细胞泌酸小管功能。AIM To investigate the regulative mechanism of electroacupunture (EA) on gastric acid secretion in rats. METHODS SD rats ( n =75) were randomized into 5 groups: Control group, non point group, Sanyinjiao group, zasanli group, and unite group. All rats in consciousness were stimulated by electroacupuncture except control group. We measured the pH of fasting gastric juice as well as its volume and acidity. Meanwhile radioimmunoassay was applied to assay the contents of gastrin (Gas) and epidermal grouth factor (EGF) in plasma, gastric juice and gastric mucosa tissue. Cellular ultra microstructure of gastric mucosa coat was observed under a fransmission electron microscope. RESULTS After EA, it was discovered that the volume of the fasting gastric juice significantly reduced (0.30±0.11) vs (0.63±0.12) mL, P <0.01,and the acidity of the gastric juice significantly dropped (30±3) vs (41±1) mmol·L -1 , P <0.05, and the pH of gastric juice rose. EA stimulation of zusanli led to a drop of plasmic Gas (84±22) vs (108±30) ng·L -1 , P <0.01, a rise of Gas in gastric mucous coats (136±17) vs (73±4) ng·g -1 , P <0.01, but little change in Gas in gastric juice. Besides, there were some other changes observed. EGF of both gastric juice and gastric mucous coat increased (3.2±1.1) vs (1.8±0.4) μg·g -1 , P <0.01, and (5.1±0.8) vs (2.4±0.4) μg·g -1 , P <0.01 respectively wheres plasmic EGF didn't change, were unfilled and didn't decrease in their number. The above indexes in non point group were not different from those of control group ( P < 0.05 ). CONCLUSION EA stimulation of zusanli can inhibit Gas release, reduce Gas contents in plasma and gastric juice, enhance contents of EGF in gastric mucosa and juice, change ultra microstructure of gastric gland cells, and inhibit the secretory function of acinotubules in paritetal cell. Finaly, The gastric acid secretion is decreased.

关 键 词：电针 足三里 胃酸 胃肠激素 

分 类 号：R245.97[医药卫生—针灸推拿学] R229[医药卫生—中医临床基础]