电针足三里对大鼠ir-SP及细胞免疫的影响  

Regulatory effects of electro acupuncture at Tsusanli on ir SP contents in rats' pituitary gland and peripheral blood and their immunity

作　　者：高巍[1] 黄裕新[1] 陈洪[2] 赵宁侠[3] 王庆莉[1] 秦明[1] 张洪新[4] 

机构地区：[1]第四军医大学唐都医院消化内科,陕西西安710038 [2]第四军医大学唐都医院医教部,陕西西安710038 [3]第四军医大学唐都医院中医科,陕西西安710038 [4]第四军医大学唐都医院介入放射科,陕西西安710038

出　　处：《第四军医大学学报》2001年第9期793-796,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目! (39970 888)

摘　　要：目的　研究电针 (EA)刺激大鼠足三里穴对免疫活性 P物质 (ir- SP)及细胞免疫的影响 .方法　将大鼠随机分成正常对照组、足三里组、免疫抑制模型组、非经非穴组、足三里+免疫抑制组 .应用放射免疫法测定脑垂体和外周血 ir- SP含量的变化 ;应用流式细胞仪技术 ,通过微量全血直接免疫荧光染色法测定外周血 T细胞亚群以反映细胞免疫功能 ;应用红细胞 C3b受体 -酵母菌花环试验和红细胞 - IC花环试验检测红细胞免疫功能 .结果　免疫抑制模型组大鼠脑垂体和外周血中 ir- SP含量、外周血的 CD4+ (P<0 .0 1) ,RBC- C3b RR(P<0 .0 5 )明显低于正常对照组 ,CD8+ 无显著变化 (P>0 .0 5 ) ,足三里组 +免疫抑制组各项指标与正常对照组相比无显著差异(P>0 .0 5 ) .电针刺激足三里穴后正常大鼠脑垂体和外周血中 ir- SP含量、外周血的 CD4+、RBC- C3b RR、RBC- ICR均明显升高 (P<0 .0 1) ,CD8+无显著变化 (P>0 .0 5 ) ,且脑垂体和外周血中 ir- SP含量与 CD4+ 、CD4+ 和 RBC- C3b RR呈显著正相关 (r=0 .744 ,P<0 .0 5 ;r=0 .738,P<0 .0 5 ;r=0 .719,P<0 .0 5 ) .非经非穴组的各项指标与正常对照组相比无显著差异(P>0 .0 5 ) .结论　电针刺激足三里穴可提高正常大鼠及免疫抑制模型大鼠的细胞免疫功能和红细胞免疫粘附功能 ,AIM To reveal the influence of electro acupuncture (EA) both on the contents of substance P in centrum and peripheral blood and on its immune function in rats. METHODS The SD rats were divided into five groups, including control group, Zusanli group, immunosuppressive model group, none point group and immunosuppressive model+EA group. The contents of ir SP in centrum and peripheral blood were determined by Radioimmunoassay. Also T lymphocyte subgroups of peripheral blood (including CD 4 + and CD 8 +) were measured by direct microvolume whole blood immunoflurescent staining technique with flow cytometry (FCM) and erythrocyte immune function (including RBC C 3b RR and RBC ICR) were observed to evaluate the immune functions of rats from different aspects. RESULTS ①The contents of ir SP in centrum and peripheral blood, the value of CD 4 +, RBC C 3b RR and RBC ICR in EA group were significantly higher than those of control group ( P <0.01), and the value of CD 8 + was not statistically different between two groups. ②The variation of CD 4 + was significantly related with RBC C 3b RR ( r =0.744, P <0.05; r =0.738, P <0.05; r =0.719, P <0.05). ③ The contents of ir SP ( P <0.01)in centrum and peripheral blood, the value of CD 4 + ( P <0.05) and RBC C 3b RR ( P <0.01) in immunosuppressive model group were observably lower than those of control group, and the value of CD 8 + was not statistically different between two groups( P >0.05), which indicated that CY restrained the T lymphocyte subgroups of peripheral blood and erythrocyte immune function. ④ In immunosuppressive model+EA group, there was no marked difference as compared with control group ( P >0.05), while there was statistical difference as compared with immunosuppressive model group ( P < 0.01 ). ⑤The date of the non Tsusanli group was not different from control group ( P >0.05). CONCLUSION When EA improves cellular immunity induced by T lymphocyte and red blood cell immune

关 键 词：针刺 足三里 大鼠 P物质 免疫 红细胞 T细胞亚群 

分 类 号：R245.97[医药卫生—针灸推拿学] R246[医药卫生—中医临床基础]