协同凝集试验检测O1群霍乱弧菌  

Coagglutination test for detecting V. Cholerae serotype O1

作　　者：陈兆鹏[1] 袁应华[1] 刘忠明 

机构地区：[1]同济大学医学院附属同济医院检验科,上海200065 [2]上海市普陀区卫生防疫站,上海200062

出　　处：《上海医学检验杂志》2001年第3期136-137,共2页Shanghai Journal of Medical Laboratory Sciences

摘　　要：目的建立一种快速简便的 O1群霍乱弧菌检测方法。方法将抗 O1群霍乱弧菌多价单克隆抗体包被到金黄色葡萄球菌 Cowan I株上制成 SPA菌体试剂 ,建立起协同凝集试验 (COA) ,检测粪便标本 6 h培养物中的霍乱弧菌。结果 COA检测的敏感性为 4× 10 6 CFU/ m l,检测过程为 5 min。 92份临床标本的测定结果与培养法相比较 ,COA的特异性、敏感性、诊断符合率分别是 10 0 %、95 .5 %、97.8%。结论 COA简便、快速 。Objective To develop a simple and rapid method for detecting V. cholerae O1. Methods Staphylococcus aureus Cowan I was coated with anti V. cholerae O1 monoclonal antibodies and a coagglutination test(COA) detecting V. cholerae O1 in 6 h fecal enrichment cultures was tested. Results The detect limit of COA was 4×10 6 CFU/ml, and detect time was 5 min. When 92 samples were tested, the diagnostic specificity, sensitivity and accuracy of the test, compared with standard culture methods, were 100%、95 5%、97 8%,respectively. Conclusion The COA is simple and less time consuming, and can be used as a new method for rapid detection of V. Cholerae.

关 键 词：霍乱弧菌 协同凝集试验 检测 霍乱 诊断 

分 类 号：R516.5[医药卫生—内科学] R378.3[医药卫生—临床医学]