人参皂甙Rg1和Rb1对骨髓粒-巨噬系祖细胞增殖的作用  

Effects of Ginsenosides Rg1 and Rb1 on Proliferation of Human Marrow Granulocyte-Macrophage Progenitor Cells

作　　者：牛泱平[1] 金锦梅[1] 高瑞兰[1] 谢桂丽[1] 陈小红[1] 

机构地区：[1]浙江省中医院血液病研究室,杭州310006

出　　处：《中国实验血液学杂志》2001年第2期178-180,共3页Journal of Experimental Hematology

摘　　要：用体外造血祖细胞集落形成技术 ,观察人参皂甙Rg1和Rb1对正常人粒 巨噬系祖细胞(CFU GM )的刺激增殖作用。实验结果表明 :Rg1浓度为 2 .5 ,5 ,10和 5 0 μg ml时CFU GM集落产率均显著高于对照组 (P <0 .0 1) ,提高率分别为 (39.8± 3.6 ) % ,(70 .6± 6 .8) % ,(6 3.0± 6 .4 ) %和 (6 0 .8± 5 .9) %。Rb1浓度为 0 .5 ,2 .5 ,5 ,10和 5 0 μg ml时CFU GM集落产率均明显高于对照组 (P <0 .0 5 ) ,提高率分别为 (2 4 .5± 2 .3) % ,(45 .6± 4 .7) % ,(5 4.6± 6 .1) % ,(6 5 .1± 6 .3) %和(5 8.3± 5 .7) %。高浓度 (5 0 μg ml)不显示抑制作用。上述结果说明 ,Rg1和Rb1对CFU GM具有明显的刺激增殖作用 。Ginseng is a traditional Chinese medicine which has been used in treating anemia for thousands of years. It is composed of a lot of components. The main component is total saponin of panax ginseng(TSPG), which contains more than 20 ginsenosides including Rg1, Rb1 and so on. Previous studies have reported that total saponin of panax ginseng could promote hematopoiesis by stimulating proliferation of human erythroid grogenitor cells CFU E and BFU E, however, it had different effects on CFU GM reported by various laboratories. In this study, CFU GM assay was adopted to observe the ginsenosides Rg1 and Rb1′s effects on the proliferation of human marrow grannulocyte macrophage progenitor cells. The results showed that Rg1 and Rb1 had obvious promotive effect on the proliferation of CFU GM, and the increasing rates of colony formation were up to (70.6±6.8)% and (65.1±6.3)%, respectively. There was no inhibiting effect on CFU GM in high concentrations of Rg1 and Rb1. It is suggested that Rg1 and Rb1 can stimulate the proliferation of human granulocyte macrophage progentors. The results of TSPG′s various effects on CFU GM might be caused by different contents of ginsenosides in TSPG used in different laboratories.

关 键 词：人参皂甙RG1 人参皂甙RB1 粒-巨噬系祖细胞 细胞增殖 

分 类 号：R973[医药卫生—药品] R285[医药卫生—药学]