人脐带血CD34^+细胞体外诱导树突状细胞及其激活T细胞抗肿瘤免疫反应的实验研究  

Generation of Dendritic Cells from Human Cord Blood CD34^+ Cells and Their Activation of T Cell-Dependent Antitumor Immune Response In Vitro

作　　者：王冠军[1] 解丽华[1] 王晓[2] 冯凯[2] 李梁[2] 裴雪涛[2] 

机构地区：[1]吉林大学第一医院血液肿瘤科,长春130021 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《中国实验血液学杂志》2001年第2期188-190,共3页Journal of Experimental Hematology

基　　金：国家杰出青年科学基金资助 编号 3982 5 111

摘　　要：To induce the growth and differentiation of dendritic cells (DCs) from human cord blood, CD34 + cells isolated from human cord blood by mini MACS were cultured in a liquid culture system with rhSCF, rhGM CSF, rhTNF α and rhFL for 10 days. Then the induced cells were characterized by DC′s morphological and phenotypic properties. In addition, they stimulated the proliferation of allogeneic T cells and possessed an efficient capacity for initiating T cell dependent antitumor immune responses in vitro. It is concluded that mature DCs could be obtained from human cord blood CD34 + cells.To induce the growth and differentiation of dendritic cells (DCs) from human cord blood, CD34 + cells isolated from human cord blood by mini MACS were cultured in a liquid culture system with rhSCF, rhGM CSF, rhTNF α and rhFL for 10 days. Then the induced cells were characterized by DC′s morphological and phenotypic properties. In addition, they stimulated the proliferation of allogeneic T cells and possessed an efficient capacity for initiating T cell dependent antitumor immune responses in vitro. It is concluded that mature DCs could be obtained from human cord blood CD34 + cells.

关 键 词：脐带血 CD34^+细胞 树突状细胞 T细胞 抗肿瘤免疫反应 实验研究 

分 类 号：R331.1[医药卫生—人体生理学] R392.11[医药卫生—基础医学]