抗病毒1号中药对流感病毒A_3的抑制作用  

The anti-influenza effect of a Chinese medicinal herb-Antiviral Agent No. 1 in MDCK culture

作　　者：杨怡姝[1] 李洪源[1] 刘密凤[1] 刘电力[1] 周昆[1] 佟奎明[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院流行病学教研室,黑龙江哈尔滨150001

出　　处：《疾病控制杂志》2001年第2期102-104,共3页Chinese Journal of Disease Control and Prevention

摘　　要：目的　了解抗病毒 1号单味中草药对流感病毒 A3的抑制作用。方法　采用细胞培养技术 ,以病毒唑为阳性对照药 ,观察抗病毒 1号对流感病毒的抑制作用。结果　抗病毒 1号半数中毒浓度 (TC5 0 )为 6 0 .5 3m g· ml- 1 ;治疗和预防给药方式均有抗流感病毒作用 ,其抗流感病毒 A3的半数有效浓度 (EC5 0 )分别为 5 .14mg· m l- 1和 5 .2 0 m g· ml- 1 ;治疗指数 (TI)分别为 11.78和 11.6 5 ;抗病毒 1号对流感病毒 A3的抑制作用存在着明显的量效反应关系 (P <0 .0 1)。结论　抗病毒 1号在 MDCK细胞中对流感病毒 A3有抑制作用。Objective To observe the anti influenza effect of a Chinese medicinal herb Antiviral Agent No.1. Methods To study the anti viral effect of Antiviral Agent No.1 by means of the technique of cell culture and using Ribavirin as a positive control. Results In MDCK culture, Antiviral Agent No.1 was found to be a potential inhibitor of influenza A 3 virus in a concentration dependent manner, with a TC 50 of 60.53 mg·ml -1 . When drug was added 2 hours post virus infection, the EC 50 (TI) was 5.14 mg·ml -1 (11.78); while drug was added 2 hours before infection, the EC 50 (TI) was 5.20 mg·ml -1 (11.65). Conclusions Antiviral Agent No.1 had a significant anti influenza effect on type A 3 in MDCK.

关 键 词：流感病毒 抗病毒作用 中草药 抗病毒1号 流行性感冒 

分 类 号：R286.87[医药卫生—中药学] R285[医药卫生—中医学]