HPLC法测定复方替硝唑漱口液中替硝唑和呋喃西林的含量  

Contents Determination of Metronidazole and Furacilin in its Collut with HPLC Method

作　　者：李冬梅[1] 李红秋[2] 魏萍[3] 

机构地区：[1]中国人民解放军251医院药材科,河北张家口075000 [2]中国人民解放军263医院药材科,北京101149 [3]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《解放军药学学报》2001年第4期180-181,184,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　建立测定复方替硝唑漱口液中替硝唑、呋喃西林含量的高效液相色谱法。方法　采用反相ODS色谱柱 ,水 -乙腈 -冰醋酸 (85∶15∶0 .1)为流动相 ,检测波长为 317nm。结果　替硝唑和呋喃西林的线性范围分别为 5 0～ 35 0 μg·ml-1(r=0 .9996 ) ,10～ 70 μg·ml-1(r =0 .9997) ;加样回收率分别为 10 0 .5 6 %、RSD =0 .6 9% ,10 0 .6 4%、RSD =0 .79% ;日内RSD为 0 .12 %～ 1.35 %和 0 .6 6 %～ 1.45 % ,n =6 ;日间RSD为 0 .2 8%～ 1.39%和 0 .18%～ 1.2 3% ,n =5。结论　HPLC法可用于复方替硝唑漱口液的含量测定 ,方法准确、灵敏 。Aim To establish a HPLC method for simultaneous determination of metronidazole and furacilin in its Collut.Method An ODS column was used and the mixture of acetonirile-water-acetic acid glacial(15∶85∶0.1)was used as mobile phase,the detection wavelength was 317nm.Results The linear ranges of metrondazole and furacilin were 50～350μg·ml -1 (r=0.999 6) and 10～70μg·ml -1 (r=0.999 7),respectively.The average added sample recovery were 100.56%,RSD was 0.69% and 100.64%,SD was 0.79% respectively.The RSD of intea-day of two were 0.12%～1.35% and 0.66%～1.45%,n=6.The RSD of inter-day of two constituents were 0.28%～1.39% and 0.18%～1.23%,n5.Conclusion The method is accurate, sensitive for compound metrondazole collut.

关 键 词：高效液相色谱法 替硝唑 呋喃西林 濑口液 含量 测定 

分 类 号：R927.2[医药卫生—药学]