蚯蚓毒素及其去除方法的初步研究  

A preliminary study on the toxin of earthworm and the methods to take it off

作　　者：张祖珣[1] 何俐明[1] 于培兰[1] 杨典洱[1] 刘华[1] 

机构地区：[1]首都医科大学生化教研室,北京100054

出　　处：《中国生化药物杂志》2001年第3期112-113,共2页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的　研究蚯蚓毒素的来源及其成分 ,探索其去除方法。方法对蚯蚓应激分泌液进行透析、可见及紫外分光光度法检测透析袋内外液组分、电泳分析蛋白组分、动物体内试验检测毒性。结果蚯蚓应激分泌液的蛋白组分显示剧毒性 (最小致死量为 0 .2 42mg/kg)。PAGE分离主要有两条带 ;蚯蚓对不同刺激产生的应激分泌液成分相同。结论蚯蚓应激分泌液的蛋白组分有毒 ,用加盐或微电流刺激方法可去除毒素。PurposeThe source of earthworm toxin, its ingredients and the methods to take it off were studied.MethodsThe irritable secretor was dialyzed, and then examined by UV-Vis spectrum instrument.The proteins was analyzed with electrophoresis. The toxicity was tested in vivo.ResultsThe proteins of irritable secretor 0.242mg/kg iv cause a mouse to death.Many bands were separated from the proteins with SDS-PAGE, among which two kinds were the principal. Even with different stimulus the secretor of earthworm was identical in ingredients.ConclusionThe results demonstrated that the earthworm proteins of irritable secretion had toxicity and the toxin could be removed from earthworm by adding NaCl or 40V electricity.

关 键 词：蚯蚓 应激分泌液 毒性蛋白 中药 

分 类 号：R282.74[医药卫生—中药学]