凝血酶的固定化及其稳定性研究  

Study on immobilization and stability of thrombin

作　　者：李平[1] 任淑玲[2] 张亿[2] 韦萍 

机构地区：[1]兰州医学院第一附属医院,甘肃兰州730000 [2]甘肃省药品检验所,甘肃兰州730000 [3]兰州市第一人民医院,甘肃兰州730050

出　　处：《中国生化药物杂志》2001年第3期134-136,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：甘肃省卫生厅资助课题 (QS981 B3 3 2 0 )

摘　　要：目的研制稳定性较高的固定化凝血酶。方法以壳聚糖 (0 .6g)为载体 ,0 .1%戊二醛 0 .3ml为交联剂 ,考察 0 .15g(10 0 0u)凝血酶的固定化条件及固定化凝血酶的稳定性。结果所制固定化凝血酶回收率达 87%以上 ,稳定性明显提高 ,制备工艺可行。结论固定化条件正确合理 ,固定化凝血酶在光照、高温、低温和室温留样观察条件下显示较好的稳定性。PurposeThe aim is to immobilize the thrombin and to increase the stability of immobilized enzyme.MethodsThrombin was immobilized on chitosan and crosslinked with glutaraldehyde, and the optimum conditions for immobilization and stability of immobilized enzyme were studied.ResultsThis preparation of immobilized thrombin was practicable,and the stability of this immobilized enzyme was obviously improved than that of thrombin.ConclutionThe conditions for immobilization were good, and reliable.Stability of immobilized thrombin to light, high temperature,low temperature and observation on reserving at room temperature was good.

关 键 词：凝血酶 壳聚糖 固定化 稳定性 

分 类 号：R977.3[医药卫生—药品] R973.1[医药卫生—药学]