胎盘提取物促淋巴细胞增殖活性及稳定性实验初探  

Initial experimental research on placenta extract to stimulate lymphocyte proliferation activity and stability

作　　者：王国华[1] 程往太 马红燕[3] 陈志阳[1] 药立波[1] 

机构地区：[1]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710032 [2]阳城县人民医院,山西阳城048100 [3]第四军医大学西京医院血库,陕西西安710032

出　　处：《中国生化药物杂志》2001年第3期137-139,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的从人胎盘组织制备促进免疫细胞增殖提取物并寻求保存提取物的适宜条件。方法通过热处理胎盘匀浆液制备提取物 ,并用溴化四唑蓝染料结合法测定体外促小鼠脾淋巴细胞的增殖活性 ,同时对提取物进行放射性同位素60 Co照射。结果经Lowry′s法测定为每 1g胎盘组织含 2～ 3mg蛋白质 ,其促细胞增殖率 >80 % ,经同位素处理后低温保存数月活性不受影响。结论此法制备的提取物不仅活性高 ,且方法简便、重复性好 ,该提取物作为一种稳定、可靠、明显的促淋巴细胞增殖反应具有上规模开发、生产、应用于临床的价值。PurposeThe aim is to prepare the extract of immunocompetent cell proliferation from human placenta and to try to find out a suitable method of preserving the extract.MethodsThe extract was mainly prepared by heat-treating placenta homogenated fluid. Then the activity to stimulate murine splenic lymphocyte proliferation in vitro was done with MTT , exposingtheextract toradioisotope 60Co.ResultsThe content of protein was 2～3mg per gram placenta measured by Lowry′s Method.The rate to promote cell proliferation was more than 80 percent.The activity lasted a few months after being exposed to radioisotope.ConclusionThe extract prepared by heat-treating not only had high activity, but also had the unique method and good repeatability.This prepared extract as a kind of stable,reliable and remarkably promoting lymphocyte proliferation reaction had the value of development production on broad scale as well as in clinical practice.

关 键 词：溴化四唑蓝 淋巴细胞增殖反应 胎盘提取物 放射性同位素^60Co 

分 类 号：R392－33[医药卫生—免疫学]